Synthesis of Biogenic Silver Nanoparticles (AgCl-NPs) Using a Gaertn. Aerial Part Extract and Their Application as Antibacterial, Antifungal and Antioxidant Agents.

In this study, very simple and fast one-step synthesis of biogenic silver chloride nanoparticles (AgCl-NPs) using a Gaertn. aerial part extract from an aqueous solution of silver nitrate at room temperature is proposed. The proceedings of the reaction were investigated by UV-Vis spectroscopy. AgCl-NPs were characterized using X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). Antibacterial and antifungal activities of these nanoparticles were evaluated by disk diffusion and microdilution methods against , , and . In addition, the antioxidant activity of the synthesized AgCl-NPs was determined by the DPPH radical scavenging assay. The antimicrobial test confirmed the bactericidal activity of biosynthesized AgCl-NPs against Gram-positive and Gram-negative bacteria. They also exhibited good antifungal activities with minimum inhibitory concentration (MIC) values ranging from 40 to 60 µg/mL against and , respectively. In addition, biosynthesized AgCl-NPs were established to have remarkable antioxidant activity. All this pointed out that the proposed new biosynthesis approach resulted in production of AgCl-NPs with convenient biomedical applications.