Proteome analysis of male accessory gland secretions in Leucinodes orbonalis Guenee (Lepidoptera: Crambidae), a Solanum melongena L. pest.

Male accessory gland (MAG) proteins are transferred along with the sperm to females at the time of mating and have diverse effects on female reproductive physiology in a wide range of insects. In this study, we sought to identify the MAG proteins in Leucinodes orbonalis Guenee, a Solanum melongena L. pest, by analyzing the MAG proteins of virgin and mated male moths by nano-LC-ESI-MS/MS techniques. A total of 142 and 131 proteins in virgin and mated males were identified, respectively, among which 17 (12.0%) and 10 (7.6%) proteins were found to show secretory signals in virgin and mated males, respectively. These secretory proteins were shown to be involved in several biological processes in insects, including egg development, sperm-related functions/capacitation, defense, metabolism, and protein chaperoning. To the best of our knowledge, this is the first study to perform a proteome analysis of the MAG proteins of L. orbonalis, and offers an opportunity for further investigation of the functions of these proteins. In insects, certain MAG proteins are known to inhibit mating whereas others accelerate egg-laying. Therefore, the identification of these proteins in L. orbonalis may be useful for pest control.