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Internalized FGF-2-Loaded Nanoparticles Increase Nuclear ERK1/2 Content and Result in Lung Cancer Cell Death. | LitMetric

Internalized FGF-2-Loaded Nanoparticles Increase Nuclear ERK1/2 Content and Result in Lung Cancer Cell Death.

Nanomaterials (Basel)

Bioengineering Program, College of Engineering and Mathematical Sciences, Larner College of Medicine, College of Engineering and Mathematical Sciences, University of Vermont, Burlington VT 05405, USA.

Published: March 2020

AI Article Synopsis

  • - Innovative cancer treatments using nanoparticles could enhance therapy and minimize side effects by enabling controlled delivery of biomolecules without needing external signals.
  • - The study focused on encapsulating basic fibroblast growth factor (FGF-2) in alginate-based nanoparticles to see how its delivery affects cancer cell growth and survival, particularly in a specific type of lung cancer cell line (A549).
  • - Results showed that delivering FGF-2 via these nanoparticles increased cancer cell death by boosting the activation of a signaling molecule (ERK1/2), while non-cancerous cells were largely unaffected, highlighting the potential for targeted cancer therapies.

Article Abstract

Innovative cancer treatments, which improve adjuvant therapy and reduce adverse events, are desperately needed. Nanoparticles provide controlled intracellular biomolecule delivery in the absence of activating external cell surface receptors. Prior reports suggest that intracrine signaling, following overexpression of basic fibroblast growth factor (FGF-2) after viral transduction, has a toxic effect on diseased cells. Herein, the research goals were to 1) encapsulate recombinant FGF-2 within stable, alginate-based nanoparticles (ABNs) for non-specific cellular uptake, and 2) determine the effects of ABN-mediated intracellular delivery of FGF-2 on cancer cell proliferation/survival. In culture, human alveolar adenocarcinoma basal epithelial cell line (A549s) and immortalized human bronchial epithelial cell line (HBE1s) internalized ABNs through non-selective endocytosis. Compared to A549s exposed to empty (i.e., blank) ABNs, the intracellular delivery of FGF-2 via ABNs significantly increased the levels of lactate dehydrogenase, indicating that FGF-2-ABN treatment decreased the transformed cell integrity. Noticeably, the nontransformed cells were not significantly affected by FGF-2-loaded ABN treatment. Furthermore, FGF-2-loaded ABNs significantly increased nuclear levels of activated-extracellular signal-regulated kinase ½ (ERK1/2) in A549s but had no significant effect on HBE1 nuclear ERK1/2 expression. Our novel intracellular delivery method of FGF-2 via nanoparticles resulted in increased cancer cell death via increased nuclear ERK1/2 activation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221911PMC
http://dx.doi.org/10.3390/nano10040612DOI Listing

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