sp. nov., isolated from ginseng cultivation soil.

A Gram-stain-negative, strictly aerobic, motile, ivory-coloured and rod-shaped bacterium (designated Gsoil 520) isolated from ginseng cultivation soil was characterized by using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 520 was observed to grow optimally at 30 °C and pH 7.0 on Reasoner's 2A agar medium. The results of phylogenetic analysis, based on 16S rRNA gene sequence similarities, indicated that Gsoil 520 belongs to the genus of the family and was most closely related to E84 (98.0 %), Yak96B (97.7 %), J1 (97.7 %) and IPL18 (96.8 %). The complete genome of strain Gsoil 520 is a presumptive circular chromosome of 4 480 314 base pairs having G+C content of 63.7 mol%. A total of 4 354 genes, 4 303 CDS and 43 rRNA genes were assigned a putative function. The major isoprenoid quinone was Q-10. The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminolipids (AL1 and AL3). The predominant fatty acids of strain Gsoil 520 were Cω7 11-methyl, C and Cω7/Cω6 (summed feature 8) supporting the affiliation of strain Gsoil 520 to the genus . The low values of DNA-DNA hybridization distinguished strain Gsoil 520 from the recognized species of the genus . Thus, the novel isolate represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain Gsoil 520 (=KACC 19440=LMG 30329).