Biofilm formation is a harmful phenomenon in many areas, such as in industry and clinically, but offers advantages in the field of biocatalysis for the generation of robust biocatalytic platforms. In this work, we optimised growth conditions for the production of Escherichia coli biofilms by three strains (PHL644, a K-12 derivative with enhanced expression of the adhesin curli; the commercially-used strain BL21; and the probiotic Nissle 1917) on a variety of surfaces (plastics, stainless steel and PTFE). E. coli PHL644 and PTFE were chosen as optimal strain and substratum, respectively, and conditions (including medium, temperature, and glucose concentration) for biofilm growth were determined. Finally, the impact of these growth conditions on expression of the curli genes was determined using flow cytometry for planktonic and sedimented cells. We reveal new insights into the formation of biofilms and expression of curli in E. coli K-12 in response to environmental conditions.
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http://dx.doi.org/10.1007/s00203-020-01864-5 | DOI Listing |
Plant Cell Environ
January 2025
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China.
In natural environments, the growth and development of trees are continuously affected by phosphorus (P) starvation stress. However, the mechanisms through which trees balance stem growth and P distribution remain unknown. This study found that in the woody model species poplar, the P loss in stems is more severe than that in roots and leaves under P starvation conditions, thereby inhibiting stem development and reducing the expression of numerous genes related to wood formation, including PagSND1-B1.
View Article and Find Full Text PDFVet Dermatol
January 2025
IVC Evidensia Referral Hospital, EDZ Arnhem, Arnhem, the Netherlands.
Background: Alopecia X (AX) is a common noninflammatory alopecic condition of Pomeranian dogs with an unknown cause. While a genetic aetiology is suspected, no current tests can predict the development of this disorder or confirm the aetiology. Therefore, identifying potential risk indicators for the development of AX would be of value in this breed.
View Article and Find Full Text PDFGlob Chang Biol
January 2025
Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Stomata control plant water loss and photosynthetic carbon gain. Developing more generalized and accurate stomatal models is essential for earth system models and predicting responses under novel environmental conditions associated with global change. Plant optimality theories offer one promising approach, but most such theories assume that stomatal conductance maximizes photosynthetic net carbon assimilation subject to some cost or constraint of water.
View Article and Find Full Text PDFJ Dent Sci
January 2025
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Background/purpose: Salivary microbiome has become a surrogate indicator of oral disease due to its collective reservoirs and convenience in sampling. However, failed clinical trials often lead to wastes of resources, indicating a need for preclinical models. In this pilot study, we aimed to compare the salivary microbiome by metagenomics analysis before and after lysogeny broth culture for prospective translational studies.
View Article and Find Full Text PDFJ Dent Sci
January 2025
Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
Background/purpose: Various pulp-covering materials offer advantages in regenerative root canal treatment, but each has limitations, highlighting the need for more effective antibacterial strategies for pulp repair and regeneration. Mesoporous bioactive glasses (MBG) show significant biological activity, making them valuable in tissue/dental repair. Silver-incorporated MBG exhibits promising antibacterial effects against various bacteria; copper ions are crucial in regulating angiogenesis signals.
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