AI Article Synopsis
- High-throughput sequencing of cDNA (RNASeq) is the preferred method for studying transcriptomes in kinetoplastids.
- This chapter covers key factors for designing RNASeq experiments, whether using cultured cells or animal models.
- It includes protocols for collecting parasites from rodents, removing rRNA from total RNA, and offers custom pipelines for sequence alignment and data analysis.
Article Abstract
High-throughput sequencing of cDNA (RNASeq) is now the method of choice for analysis of transcriptomes. This chapter details important considerations in the design of RNASeq experiments for kinetoplastids grown in culture or experimental animals. It contains protocols for obtaining parasites from rodents, and for removal of rRNA from total RNA. In addition, custom pipelines for sequence alignment, and for data analysis and visualization, are described.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-0294-2_6 | DOI Listing |
Publication Analysis
Top Keywords
high-throughput sequencing
8
sequencing trypanosome
4
trypanosome transcriptome
4
transcriptome characterization
4
characterization high-throughput
4
sequencing cdna
4
cdna rnaseq
4
rnaseq method
4
method choice
4
choice analysis
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!