The infection of triatomines with trypanosomes can be performed with different forms of the parasite, and the procedure is important not only for vector-parasite interaction studies but also for maintaining the infectivity of parasite strains, which guarantees more realistic biological and molecular investigations. Here, I describe how to infect the vector Rhodnius prolixus, a model species, with two different species of Trypanosoma.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/978-1-0716-0294-2_5 | DOI Listing |
PLoS Negl Trop Dis
January 2025
Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, South Carolina, United States of America.
Reactivation of Trypanosoma cruzi transmission by native vectors with different domiciliation capabilities is a major concern for Chagas disease control programs. T. cruzi transmission via intra-domestic Rhodnius prolixus was certified as interrupted by the Pan American Health Organization in Miraflores municipality (Boyacá, Colombia) in 2019.
View Article and Find Full Text PDFMicroorganisms
January 2025
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, Mexico.
The primary mode of transmission for Chagas disease is vector-borne transmission, spread by hematophagous insects of the subfamily. In Mexico, the triatomine is particularly significant in the transmission of . This study focused on analyzing protein expression and modifications by glycosylation in different regions of the digestive tract of fifth-instar nymphs of .
View Article and Find Full Text PDFEcohealth
January 2025
Laboratorio de Medicina y Endocrinología de la Fauna Silvestre, IMBECU, UNCuyo - CONICET, Av. Dr. Adrian Ruiz Leal s/n, Parque General San Martín, Mendoza, Argentina.
Urban domestic dog populations can provide important clues about the eco-epidemiological characteristics of Trypanosoma cruzi, the causative agent of Chagas disease (ChD). Given the limited data on ChD from the Metropolitan Area of Mendoza, Argentina, a seroprevalence survey of 327 dogs across an urban-rural gradient was conducted between April 2018 and May 2019. Seropositive cases were analyzed considering host, social, and environmental factors, subtypes (DTUs), and bloodstream parasite load.
View Article and Find Full Text PDFCells
December 2024
Department of Biological Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
The hemoflagellate parasite is transmitted by triatomine kissing bugs and may co-infect humans together with its Chagas disease-causing congener . Using real-time quantitative polymerase chain reaction (RT-qPCR) and antimicrobial assays, we studied () the temporal and spatial distribution of in common bed bugs, , following oral ingestion and hemocoelic injection of and () the immune responses of bed bugs induced by infections. Irrespective of infection mode, no live were present in the bed bugs' hemolymph, salivary glands, or feces.
View Article and Find Full Text PDFG3 (Bethesda)
December 2024
Department of Entomology and Wildlife Ecology, University of Delaware, Newark, DE 19716, USA.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!