The method of circular dichroism (CD) has been used to investigate the reconstitution of mononucleosomes from C3HA mice liver and ascitic hepatoma 22A cells chromatin. It has been revealed that the more unfolding state of DNA in ascitic nucleosomes (discovered earlier) is determined by the peculiarities of the interactions between DNA and the dimers H2A-H2B, as well as by the linker histones of the H1 group. The investigation of the DNA folding in the oligonucleosome chains with increasing ionic strength has shown complete invariability of the DNA compactness in the ascitic chromatin up to 100 mM NaCl, while in liver nucleosomes an additional folding of the linker portion of the DNA was observed within the range of 20-40 mM NaCl. Oligonucleosomes from ascitic chromatin are less inclined to association upon increasing ionic strength, as compared with those from liver chromatin.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hH1).
Protein Sci
February 2025
Cell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Purifying membrane proteins has been the limiting step for studying their structure and function. The challenges of the process include the low expression levels in heterologous systems and the requirement for their biochemical stabilization in solution. The human voltage-gated proton channel (hH1) is a good example of that: the published protocols to express and purify hH1 produce low protein quantities at high costs, which is an issue for systematically characterizing its structure and function.
View Article and Find Full Text PDFRemoval of ciprofloxacin using polymeric nanocomposites synthesized from alkylated chitosan ionic macromonomers, ionic monomers and hydrotalcite.
Int J Biol Macromol
January 2025
Polymers Department, Faculty of Chemical Sciences, University of Concepción, Box 160-C, Concepción 4070371, Chile. Electronic address:
The contamination of water systems by antibiotics such as ciprofloxacin (CIP), which is used to treat bacterial infections, poses severe risks to environmental safety and public health. To address this issue, a novel zwitterionic polymeric nanocomposite (PNs-HTC) was developed in this study. This novel material was synthesized using alkylated chitosan ionic macromonomers, ionic monomers and combined with hydrotalcite (HTC) via in situ free radical polymerization.
View Article and Find Full Text PDFA bio-based alginate hydrogel with considerable thermoelectric performance, mechanical strength and flame retardancy for ultra-fast and sustained early fire-alarm system.
Int J Biol Macromol
January 2025
School of Safety Science and Emergency Management, Wuhan University of Technology, Wuhan 430070, People's Republic of China. Electronic address:
Recently, the widespread utilization of combustible materials has increased the risks associated with building fires. Early fire-warning systems represent a pivotal strategy in mitigating losses incurred from fire incidents and offer considerable potential for the enhancement of fire safety management. This study focuses on the synthesis of bio-based ionic hydrogels, specifically calcium alginate/polyacrylamide/glycerol/lithium bromide (CPG-L), as a novel fire sensor.
View Article and Find Full Text PDFLignin-containing nanofiber-reinforced flexible strain sensors with excellent mechanical properties and ionic conductivity for human motion detection.
Int J Biol Macromol
January 2025
Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address:
A multifunctional hydrogel with outstanding mechanical properties and excellent ionic conductivity holds immense potential for applications in various fields, such as healthcare monitoring, and various devices, such as wearable devices and flexible electronics. However, developing hydrogels that combine high mechanical strength with efficient electrical conductivity remains a considerable challenge. Herein, an ion-conductive hydrogel with excellent mechanical properties and ionic conductivity is successfully created.
View Article and Find Full Text PDFMigration of vanadium oxide nanoparticles in saturated porous media.
J Hazard Mater
January 2025
MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, PR China.
Vanadium oxides nanoparticles (VO-NPs) as emerging functional materials are widely applied in high-technology industries. However, their environmental behaviors remain largely known. In this study, the migration of three common VO-NPs (VO VO, and VO) in saturated porous media has been investigated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!