Although imaging techniques using soft X-rays (SXs) are being developed as the available photon flux increases because of the continuing development of synchrotron light sources, it will be necessary to downsize the pixel size of the SX camera to produce finer SX images. Application of the stimulated emission depletion (STED) method to a scintillator plate followed by use of this plate as a sensor is one promising method to reduce the pixel size of SX cameras. A STED phenomenon occurred in the luminescence of a Ce-doped LuSiO crystal (Ce:LSO) excited using ultraviolet (UV) light when the scintillator was irradiated with azimuthally polarized laser light in the photon energy range from 1.97 eV (630 nm) to 2.58 eV (480 nm). When the excitation light source changed to synchrotron radiation (SR) light with photon energy of 800 eV, the same STED phenomenon occurred. The spot size of the luminescence was reduced by the STED phenomenon and this spot size decreased as the STED laser's photon energy increased. The energy dependence of the Ce:LSO luminescence levels can be used to explain the change in the spot size at the luminescence point.
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http://dx.doi.org/10.1038/s41598-020-62100-0 | DOI Listing |
In a previous study, the coexistence of different aggregation pathways of insulin and β-amyloid (Aβ) peptides was demonstrated by correlative stimulated emission depletion (STED) microscopy and atomic force microscopy (AFM). This had been explained by suboptimal proteins labeling strategies that generate heterogeneous populations of aggregating species. However, because of the limited number of proteins considered, the failure of the fluorescent labeling that occurs in a large portion of the aggregating fibrils observed for insulin and Aβ peptides, could not be considered a general phenomenon valid for all molecular systems.
View Article and Find Full Text PDFJ Opt Soc Am A Opt Image Sci Vis
April 2022
Spatial resolution of stimulated emission depletion (STED) microscopy varies with sample labeling techniques and microscope components, e.g., lasers, lenses, and photodetectors.
View Article and Find Full Text PDFCell Biosci
June 2021
Department of Pathology, Immunology and Laboratory Medicine, Center for Immunity and Inflammation, Rutgers University-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ, 07103, USA.
Background: The novel SARS-CoV-2 has quickly become a global pandemic since the first reported case in December 2019, with the virus infecting millions of people to date. The spike (S) protein of the SARS-CoV-2 virus plays a key role in binding to angiotensin-converting enzyme 2 (ACE2), a host cell receptor for SARS-CoV-2. S proteins that are expressed on the cell membrane can initiate receptor-dependent syncytia formation that is associated with extensive tissue damage.
View Article and Find Full Text PDFSci Rep
March 2020
Department of Electrical and Electronic Engineering, Faculty of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, 321-8585, Japan.
Although imaging techniques using soft X-rays (SXs) are being developed as the available photon flux increases because of the continuing development of synchrotron light sources, it will be necessary to downsize the pixel size of the SX camera to produce finer SX images. Application of the stimulated emission depletion (STED) method to a scintillator plate followed by use of this plate as a sensor is one promising method to reduce the pixel size of SX cameras. A STED phenomenon occurred in the luminescence of a Ce-doped LuSiO crystal (Ce:LSO) excited using ultraviolet (UV) light when the scintillator was irradiated with azimuthally polarized laser light in the photon energy range from 1.
View Article and Find Full Text PDFHNO
April 2019
Klinik für Hals‑, Nasen- und Ohrenheilkunde, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, 30625, Hannover, Deutschland.
Increasing numbers of cochlear implant patients have residual hearing. Despite surgical and pharmacological efforts to preserve residual hearing, a significant number of these patients suffer a late, unexplained loss of residual hearing. Surgical trauma can be excluded as the cause.
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