AI Article Synopsis
- Researchers identified 176 overexpressed genes in hepatocellular carcinoma (HCC) using genomic datasets, narrowing it down to 12 key genes for further validation.
- These 12 hub genes were found to promote HCC growth and spread, with their increased expression linked to advanced tumor stages and poor patient outcomes.
- The study revealed that the upregulation of these genes may occur due to hypomethylation or increased gene copies, and they also correlate with immune cell presence in HCC environments.
Article Abstract
The molecular mechanisms underlying hepatocellular carcinoma (HCC) progression remain largely undefined. Here, we identified 176 commonly upregulated genes in HCC tissues based on three Gene Expression Omnibus datasets and The Cancer Genome Atlas (TCGA) cohort. We integrated survival and methylation analyses to further obtain 12 upregulated genes for validation. These genes were overexpressed in HCC tissues at the transcription and protein levels, and increased mRNA levels were related to higher tumor grades and cancer stages. The expression of all markers was negatively associated with overall and disease-free survival in HCC patients. Most of these hub genes can promote HCC proliferation and/or metastasis. These 12 hub genes were also overexpressed and had strong prognostic value in many other cancer types. Methylation and gene copy number analyses indicated that the upregulation of these hub genes was probably due to hypomethylation or increased gene copy numbers. Further, the methylation levels of three genes, , , and , were associated with HCC clinical features. Moreover, the levels of most hub genes were related to immune cell infiltration in HCC microenvironments. Finally, we identified three upregulated genes (, , and ) that could comprehensively and accurately provide diagnostic and prognostic value for HCC patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138582 | PMC |
| http://dx.doi.org/10.18632/aging.102969 | DOI Listing |
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