AI Article Synopsis
- Advances in synthetic biology and metabolic engineering enable the creation of more efficient metabolic pathways in cell factories, bypassing the host's regulatory mechanisms.
- The research involved replacing the native MEP pathway for isoprenoid biosynthesis in Rhodobacter sphaeroides with a heterologous mevalonate (MVA) pathway from a similar bacterium.
- The engineered strain produced higher yields of sesquiterpenes than the control strain by expressing only the MVA pathway, demonstrating improved biosynthetic performance through this pathway substitution.
Article Abstract
Advances in synthetic biology and metabolic engineering have proven the potential of introducing metabolic by-passes within cell factories. These pathways can provide a more efficient alternative to endogenous counterparts due to their insensitivity to host's regulatory mechanisms. In this work, we replaced the endogenous essential 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the industrially relevant bacterium Rhodobacter sphaeroides by an orthogonal metabolic route. The native 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway was successfully replaced by a heterologous mevalonate (MVA) pathway from a related bacterium. The functional replacement was confirmed by analysis of the reporter molecule amorpha-4,11-diene after cultivation with [4- C]glucose. The engineered R. sphaeroides strain relying exclusively on the MVA pathway was completely functional in conditions for sesquiterpene production and, upon increased expression of the MVA enzymes, it reached even higher sesquiterpene yields than the control strain coexpressing both MEP and MVA modules. This work represents an example where substitution of an essential biochemical pathway by an alternative, heterologous pathway leads to enhanced biosynthetic performance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264872 | PMC |
| http://dx.doi.org/10.1111/1751-7915.13562 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
BRA-YOLOv7: improvements on large leaf disease object detection using FasterNet and dual-level routing attention in YOLOv7.
Front Plant Sci
December 2024
The Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China.
Tea leaf diseases are significant causes of reduced quality and yield in tea production. In the Yunnan region, where the climate is suitable for tea cultivation, tea leaf diseases are small, scattered, and vary in scale, making their detection challenging due to complex backgrounds and issues such as occlusion, overlap, and lighting variations. Existing object detection models often struggle to achieve high accuracy in detecting tea leaf diseases.
View Article and Find Full Text PDFCase Report: Concurrent transcatheter aortic and mitral valve replacement for symptomatic concomitant aortic and mitral valve stenoses.
Front Cardiovasc Med
December 2024
Oxford Heart Centre, Oxford University Hospitals NHS Trust, Oxford, United Kingdom.
In patients undergoing transcatheter aortic valve implantation (TAVI), multi-valve disease is common and associated with worse outcomes. Despite multiple emerging transcatheter valve treatment options, no guidelines exist for the transcatheter treatment of multi-valve disease. We present a case of a 76-year-old patient with concomitant severe aortic valve stenosis and severe mitral valve stenosis who underwent concurrent TAVI and transcatheter mitral valve replacement.
View Article and Find Full Text PDFVegetation-soil-microbiota dynamics across a 50-year reconstructed grassland chronosequence on the Loess Plateau of China.
PeerJ
December 2024
College of Forestry and Prataculture, Ningxia University, Yinchuan, China.
Alfalfa ( L.) establishment is an effective strategy for grassland reconstruction in degraded ecosystems. However, the mechanisms underlying vegetation succession in reconstructed grasslands following alfalfa establishment remain elusive.
View Article and Find Full Text PDF[Effect of percutaneous acupoint electrical stimulation on immune function and postoperative recovery in patients with total knee arthroplasty].
Zhongguo Gu Shang
December 2024
Department of Anesthesiology, Gansu Provincial Hospital of TCM, Lanzhou 730000, Gansu, China.
Objective: To evaluate clinical effect of transcutaneous acupoint electrical stimlation (TEAS) on perioperative immune function and postoperative recovery in patients with total knee arthroplasty (TKA).
Methods: From November 2021 to July 2022, 80 patients with unilateral TKA were selected and divided into TEAS group and sham TEAS group according to different treatment methods. There were 40 patients in TEAS group, including 9 males and 31 females;aged from 61 to 79 years old with an average of (66.
Corpus cavernosum and tunica albuginea reconstruction by tissue engineering: towards functional erectile structures regeneration.
BMC Urol
December 2024
Department of Cardiovascular Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, China.
Background: Current treatments for penile erectile structures reconstruction are limited and remain a great challenge in clinical practice. Tissue engineering techniques using different seed cells and scaffolds to construct a neo-tissue open promising avenues for penile erectile structures repair and replacement and show great promise in the restoration of: structure, mechanical property, and function which matches the original tissue.
Methods: A comprehensive literature review was conducted by accessing the NCBI PubMed, Cochrane, and Google Scholar databases from January 1, 1990, to January, 1, 2022 using the search terms "Tissue engineering, Corpus cavernosum (CC), Tunica albuginea (TA), Acellular Matrix, Penile Reconstruction".
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!