Scientific-grade lasers are costly components of modern microscopes. For high-power applications, such as single-molecule localization microscopy, their price can become prohibitive. Here, we present an open-source high-power laser engine that can be built for a fraction of the cost. It uses affordable, yet powerful laser diodes at wavelengths of 405 nm, 488 nm and 638 nm and optionally a 561 nm diode-pumped solid-state laser. The light is delivered to the microscope via an agitated multimode fiber in order to suppress speckles. We provide the parts list, CAD files and detailed descriptions, allowing any research group to build their own laser engine.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041445 | PMC |
| http://dx.doi.org/10.1364/BOE.380815 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Engineering Floquet Moiré Patterns for Scalable Photocurrents.
Nano Lett
January 2025
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), X5000HUA Córdoba, Argentina.
While intense laser irradiation and moiré engineering have independently proven powerful for tuning material properties on demand in condensed matter physics, their combination remains unexplored. Here we exploit tilted laser illumination to create spatially modulated light-matter interactions, leading to two striking phenomena in graphene. First, using two lasers tilted along the same axis, we create a quasi-1D supercell hosting a network of Floquet topological states that generate controllable and scalable photocurrents spanning the entire irradiated region.
View Article and Find Full Text PDFA SPR aptamer sensor for mercury based on AuNPs@NaYF:Yb,Tm,Gd upconversion luminescent nanoparticles.
Anal Methods
November 2017
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
A new aptamer-based surface plasmon resonance (SPR) system has been designed to detect Hg that utilizes near-infrared (NIR)-to-NIR gold nanoparticle coated NaYF:Yb,Tm,Gd up-conversion nanoparticles (AuNPs@NaYF:Yb,Tm,Gd UCNPs) as probes. The AuNPs@NaYF:Yb,Tm,Gd UCNPs were prepared and excited by near-infrared light (980 nm) which emitted at a near-infrared wavelength (808 nm) using an inexpensive infrared continuous wave laser diode. The AuNPs@NaYF:Yb,Tm,Gd UCNPs were conjugated with Hg aptamers.
View Article and Find Full Text PDFThermoelectric porous laser-induced graphene-based strain-temperature decoupling and self-powered sensing.
Nat Commun
January 2025
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania, 16802, USA.
Despite rapid developments of wearable self-powered sensors, it is still elusive to decouple the simultaneously applied multiple input signals. Herein, we report the design and demonstration of stretchable thermoelectric porous graphene foam-based materials via facile laser scribing for self-powered decoupled strain and temperature sensing. The resulting sensor can accurately detect temperature with a resolution of 0.
View Article and Find Full Text PDFLiquid-infused nanostructured composite as a high-performance thermal interface material for effective cooling.
Nat Commun
January 2025
Department of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA, USA.
Effective heat dissipation remains a grand challenge for energy-dense devices and systems. As heterogeneous integration becomes increasingly inevitable in electronics, thermal resistance at interfaces has emerged as a critical bottleneck for thermal management. However, existing thermal interface solutions are constrained by either high thermal resistance or poor reliability.
View Article and Find Full Text PDFActivatable Photosensitizer Prodrug for Self-Amplified Immune Therapy via Pyroptosis.
Angew Chem Int Ed Engl
January 2025
Hunan University, College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistr, 410082, Changsha, CHINA.
Immunotherapy is a promising cancer treatment, but its application is hindered by tumors' low immunogenicity and the difficulty of immune cell infiltration. Here, to address above issues and achieve targeted tumor treatment, we designed the first activated small molecule photosensitizer immune-prodrug HDIM based on pyroptosis, and proposed a self-amplified immune therapy strategy (SITS) for enhanced tumor therapy. HDIMcan be specifically activated by the tumor hypoxiaand then simultaneously initiate immuno-therapy and photodynamic therapy (PDT)-induced pyroptosis with NIR laser irradiation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!