Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138518 | PMC |
| http://dx.doi.org/10.1038/s41592-020-0782-3 | DOI Listing |
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A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.
Nat Methods
April 2020
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.
Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.
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