Shiso ( var. ) is widely grown as an important vegetable or herb crop in Japan. Beginning around the year 2000, occurrences of severe mosaic symptoms on shiso were documented and gradually spread across Kochi Prefecture, one of four major shiso production areas in Japan. Next generation sequencing and cloning indicated the presence of a previously unknown virus related to the members of the genus , for which we proposed the name Perilla mosaic virus (PerMV). The genome of PerMV consists of 10 RNA segments, each encoding a single protein in the negative-sense orientation. Of these proteins, P1, P2, P3a, P3b, P4, and P5 show amino acid sequence similarities with those of known emaraviruses, whereas no similarities were found in P6a, P6b, P6c, and P7. Characteristics of the RNA segments as well as phylogenetic analysis of P1 to P4 indicate that PerMV is a distinct and highly divergent emaravirus. Electron microscopy observations and protein analyses corresponded to presence of an emaravirus. Transmission experiments demonstrated that an eriophyid mite, sp. (family Eriophyidae), transmits PerMV with a minimum 30-min acquisition access period. Only plants belonging to the genus tested positive for PerMV, and the plant-virus-vector interactions were evaluated. The nucleotide sequences reported here are available in the DDBJ/ENA/GenBank databases under accession numbers LC496090 to LC496099.
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First report of perilla mosaic emaravirus infecting in South Korea.
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Jeonbuk National University, 26714, Graduate School on Plant Protection and Quarantine, 567 Baekje-daero, Deokjin-gu, Jeonju, Korea (the Republic of), 54896;
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- PerMV, a genus within a certain family of viruses, possesses a single-stranded RNA genome and has been identified in Japan, where it was transmitted to perilla plants by a specific type of mite.
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Department of Smart Agriculture Systems, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Republic of Korea.
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Central Region Agricultural Research Center, NARO, Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
Shiso ( var. ) is widely grown as an important vegetable or herb crop in Japan. Beginning around the year 2000, occurrences of severe mosaic symptoms on shiso were documented and gradually spread across Kochi Prefecture, one of four major shiso production areas in Japan.
View Article and Find Full Text PDF[Emergence of emaraviruses, the eriophyoid mite-transmitted viruses in plants].
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January 2008
National Institute of Agricultural Biotechnology, RDA, 225 Seodun-dong, Suwon, 441-707, South Korea.
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