AI Article Synopsis
- Researchers developed a biocatalytic method to create plant tetrahydroisoquinoline alkaloids (THIQAs) from dihydroisoquinoline (DHIQ) precursors using specific enzymes.
- They engineered an enzyme called imine reductase IR45 to enhance its ability to convert DHIQs into ()-tetrahydroisoquinolines (-THIQs) with high efficiency and selectivity.
- By combining multiple enzymes in one reaction, they established two artificial biosynthetic pathways to produce five different ()-THIQAs with a 100% yield, providing a versatile method for generating a range of plant THIQAs.
Article Abstract
Herein, we report a biocatalytic approach to synthesize plant tetrahydroisoquinoline alkaloids (THIQAs) from dihydroisoquinoline (DHIQ) precursors using imine reductases and -methyltransferase (NMT). The imine reductase IR45 was engineered to significantly expand its substrate specificity, enabling efficient and stereoselective conversion of 1-phenyl and 1-benzyl 6,7-dimethoxy-DHIQs into the corresponding ()-tetrahydroisoquinolines (-THIQs). Coclaurine -methyltransferase (CNMT) was able to further efficiently convert these ()-THIQ intermediates into ()-THIQAs. By assembling IRED, CNMT, and glucose dehydrogenase (GDH) in one reaction, we effectively constituted two artificial biosynthetic pathways in and successfully applied them to the production of five ()-THIQAs. This highly efficient (100% yield from DHIQs) and easily tailorable (adding other genes) biosynthetic approach will be useful for producing a variety of plant THIQAs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067268 | PMC |
| http://dx.doi.org/10.1039/c9sc03773j | DOI Listing |
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