Nematode caused by in the North Island brown kiwi ().

Int J Parasitol Parasites Wildl

School of Veterinary Science, Massey University, Private Bag 11-222, Palmerston North, New Zealand.

Published: April 2020

Sporadic cases of visceral and neural nematode have been diagnosed at necropsy in the endangered New Zealand kiwi ( spp.), but the causative organisms have not yet been definitively identified. From an initial group of five affected kiwi, PCR was performed on DNA extracted from archival formalin-fixed paraffin-embedded tissue sections in which larval nematodes had been histologically identified. Sequencing of positive results from four out of the five kiwi aligned with sequences from , a nematode parasite whose definitive host is the domestic cat. PCR was then performed on a second group of 12 kiwi that had histologic inflammatory lesions consistent with , but variable larval presence. Repeatable positive PCR results were only achieved in one tissue, in which larval organisms were histologically confirmed. This study supports the use of PCR as an alternative or adjunct to the morphological identification of nematode larvae in formalin-fixed histopathological samples, as well as showing that in investigation of , PCR has greatest chance of success from sections where nematode larvae are evident histologically. The identification of from lesions of in kiwi reflects an indirect, parasite-mediated effect of an invasive mammalian species on a native species.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066032PMC
http://dx.doi.org/10.1016/j.ijppaw.2020.02.011DOI Listing

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