AI Article Synopsis
- MC-LR is identified as a potent tumor initiator that causes malignant cell transformation, affecting mechanical properties crucial for cell invasion.
- The study determined that MC-LR increased cellular deformability and softness while decreasing viscoelastic parameters in DU145 and WPMY cells through micropipette aspiration techniques.
- Additionally, MC-LR caused changes in microfilaments and enhanced cell invasion, indicating that mechanical alterations and microfilament reorganization are key mechanisms in its promoted invasive behavior.
Article Abstract
Microcystin-leucine arginine (MC-LR) is a potent tumor initiator that can induce malignant cell transformation. Cellular mechanical characteristics are pivotal parameters that are closely related to cell invasion. The aim of this study is to determine the effect of MC-LR on mechanical parameters, microfilament, and cell invasion in DU145 and WPMY cells. Firstly, 10 μM MC-LR was selected as the appropriate concentration cell viability assay. Subsequently, after MC-LR treatment, the cellular deformability and viscoelastic parameters were tested using the micropipette aspiration technique. The results showed that MC-LR increased the cellular deformability, reduced the cellular viscoelastic parameter values, and caused the cells to become softer. Furthermore, microfilament and microfilament-associated proteins were examined by immunofluorescence and Western blot, respectively. Our results showed that MC-LR induced microfilament reorganization and increased the expression of p-VASP and p-ezrin. Finally, the impact of MC-LR on cell invasion was evaluated. The results revealed that MC-LR promoted cell invasion. Taken together, our results suggested that mechanical changes and microfilament reorganization were involved in MC-LR-promoted cell invasion in DU145 and WPMY cells. Our data provide novel information to explain the toxicological mechanism of MC-LR.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054891 | PMC |
| http://dx.doi.org/10.3389/fphar.2020.00089 | DOI Listing |
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