The DNA-binding protein from starved cells (Dps) is found in a wide range of microorganisms, and it has been well characterized. However, little is known about Dps proteins from nonheterocystous filamentous cyanobacteria. In this study, a Dps protein from the thermophilic nonheterocystous filamentous cyanobacterium Thermoleptolyngbya sp. O-77 (TlDps1) was purified and characterized. PAGE and CD analyses of TlDps1 demonstrated that it had higher thermostability than previously reported Dps proteins. X-ray crystallographic analysis revealed that TlDps1 possessed His-type ferroxidase centers within the cavity and unique metal-binding sites located on the surface of the protein, which presumably contributed to its exceedingly high thermostability.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327923PMC
http://dx.doi.org/10.1002/2211-5463.12837DOI Listing

Publication Analysis

Top Keywords

dps protein
8
his-type ferroxidase
8
ferroxidase centers
8
metal-binding sites
8
dps proteins
8
nonheterocystous filamentous
8
dps
5
biochemical structural
4
structural characterization
4
characterization thermostable
4

Similar Publications

Inhibition of advanced glycation end-products in frozen-thawed and reheated pork by pigskin gelatin hydrolysates.

Int J Biol Macromol

January 2025

College of Food Science and Technology, Shanghai Ocean University, No. 999 Hucheng Huan Road, LinGang New City, Shanghai 201306, China; Engineering Research Center of Food Thermal-Processing Technology, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

This study investigated the effects of pigskin gelatin hydrolysates (PGH, 4 %) as a cryoprotectant on the formation of advanced glycation end-products (AGEs) in pre-heated pork subjected to freeze-thaw cycles and subsequent reheating. During the freeze-thaw process, PGH significantly mitigated the increase in α-dicarbonyl precursors (α-DPs) and AGEs compared to the control group. Specifically, the levels of glyoxal and methylglyoxal decreased by 4.

View Article and Find Full Text PDF

Physicochemical features of subunit interfaces and their role in self-assembly across the ferritin superfamily.

Structure

December 2024

Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, NH 62, Nagaur Road, Karwar 342030, Jodhpur, Rajasthan, India. Electronic address:

Ferritins are ubiquitous and play a critical role in iron homeostasis. They are classified into four main subfamilies: classical, bacterial, bacterioferritin, and Dps. These are characterized by subunits with a four-helical bundle domain and interact through three distinct regions-one antiparallel interface (IntA) and two perpendicular interfaces (IntB and IntC), collectively forming a cage-like structure.

View Article and Find Full Text PDF

PerR functions as a redox-sensing transcription factor regulating metal homeostasis in the thermoacidophilic archaeon Saccharolobus islandicus REY15A.

Nucleic Acids Res

January 2025

CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, 266237 Qingdao, China.

Thermoacidophilic archaea thrive in environments with high temperatures and low pH where cells are prone to severe oxidative stress due to elevated levels of reactive oxygen species (ROS). While the oxidative stress responses have been extensively studied in bacteria and eukaryotes, the mechanisms in archaea remain largely unexplored. Here, using a multidisciplinary approach, we reveal that SisPerR, the homolog of bacterial PerR in Saccharolobus islandicus REY15A, is responsible for ROS response of transcriptional regulation.

View Article and Find Full Text PDF

Objectives: The aim of the present study was to investigate if major dietary patterns (DPs) interact with psychological disorders (PDs) in association with sleep quality and quantity among a large sample of Iranian adults living in Yazd, Iran.

Methods: This cross-sectional investigation was conducted on the baseline data of a population-based Iranian cohort study (Yazd Health Study-Taghzieh Mardom-e-YaZd: YaHS-TAMYZ). All data regarding dietary intakes, PDs, and sleep status were collected via validated questionnaires.

View Article and Find Full Text PDF

Germline BRCA1/2 status and chemotherapy response score in high-grade serous ovarian cancer.

Br J Cancer

December 2024

Division of Cancer Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom.

Background: High-grade serous ovarian cancer (HGSOC) can be treated with platinum-based neoadjuvant chemotherapy (NACT) and delayed primary surgery (DPS). Histopathological response to NACT can be assessed using Böhm's chemotherapy response score (CRS). We investigated whether germline BRCA1/2 (gBRCA1/2) genotype associated with omental CRS phenotype.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!