Background: Obesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Although Dlgap1 (Discs, Large (Drosophila) Homolog-Associated Protein 1) is suspected to have an effect on this process, no empirical evidence is available.
Methods: To understand the role of Dlgap1, we cultured white and brown fat cells, then performed overexpression and knockout experiments.
Results: We found that Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while also increasing brown-adipocyte proliferation and apoptosis. However, the gene overexpression has no effect on brown adipocyte maturation. Knocking out Dlgap1 in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. Additionally, the knockout inhibits white fat cell proliferation and apoptosis, while also promoting their maturation.
Conclusions: Dlgap1 negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis.
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| http://dx.doi.org/10.1186/s12944-020-01230-w | DOI Listing |
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