Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. We modified ExM with 0.06% N,N'-methylenebisacrylamide (MBAA) (termed 0.06%-MBAA ExM), increased the expansion factor to 5.7 and achieved a lateral resolution of ∼50 nm with a common confocal microscope.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d0cc00052c | DOI Listing |
Publication Analysis
Top Keywords
expansion microscopy
8
improving resolving
4
resolving ability
4
ability expansion
4
microscopy varying
4
varying crosslinker
4
crosslinker concentration
4
concentration systematically
4
systematically investigated
4
investigated performance
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!