Development of biofilm in the presence of human colostrum and 3'-sialyllactose.

Aim: To evaluate the initial adhesion and formation of biofilm in the presence of saliva, human colostrum and 3'-sialyllactose.

Methods: Human colostrum and salivas were collected from 30 mothers and newborn postpartum. Eighteen hours culture of was treated with colostrum or 3'-sialyllactose in three different moments: before, during, and after 24 h from the microbial inoculation. Salivas were also tested in conjunction with colostrum. The assays were realized in sterile 96-well flat-bottom microtiter plates for 24 h. The biofilms were fixed, washed, stained with crystal violet, and extracted. Absorbance was measured to evaluate biofilm growth mass.

Results: Colostrum applied after and during the inoculation decreased biofilm formation when compared with the control ( < .05). The presence of saliva increased the biofilm biomass ( < .05). The application of 3'-sialyllactose reduced biofilm formation independently of moments of application ( < .05).

Conclusion: Saliva contributed to the proliferation of biofilm and colostrum did not prevent the initial adhesion, but interfered in the accumulation and development of microorganisms in biofilms. 3'-sialyllactose significantly decreased biofilm formation. This information expands the importance of colostrum as a potent oral antimicrobial biofluid.