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Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system. | LitMetric

Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system.

Vet Res

Key Laboratory of Zoonosis of Ministry of Agriculture & National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.

Published: March 2020

AI Article Synopsis

Article Abstract

Eimeria tenella has emerged as valuable model organism for studying the biology and immunology of protozoan parasites with the establishment of the reverse genetic manipulation platform. In this report, we described the application of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system for efficient genetic editing in E. tenella, and showed that the CRISPR/Cas9 system mediates site-specific double-strand DNA breaks with a single guide RNA. Using this system, we successfully tagged the endogenous microneme protein 2 (EtMic2) by inserting the red fluorescent protein into the C-terminal of EtMic2. Our results extended the utility of the CRISPR/Cas9-mediated genetic modification system to E. tenella, and opened a new avenue for targeted investigation of gene functions in apicomplexan parasites.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065449PMC
http://dx.doi.org/10.1186/s13567-020-00766-0DOI Listing

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