AI Article Synopsis
- Epigenetics plays a significant role in how endothelial cells age, particularly through microRNAs that are transferred by extracellular vesicles from senescent cells.* -
- Research found that senescent endothelial cells release more small extracellular vesicles (sEVs), which are rich in specific microRNAs (miR-21-5p and miR-217) that negatively impact key genes related to cell replication and aging.* -
- Analysis of microRNAs in plasma from healthy individuals aged 40-100 revealed a pattern that supports the idea that these microRNAs can serve as biomarkers for cellular aging.*
Article Abstract
The role of epigenetics in endothelial cell senescence is a cutting-edge topic in ageing research. However, little is known of the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled by extracellular vesicles (EVs) released from senescent cells. Analysis of microRNA and DNA methylation profiles in non-senescent (control) and senescent (SEN) human umbilical vein endothelial cells (HUVECs), and microRNA profiling of their cognate small EVs (sEVs) and large EVs demonstrated that SEN cells released a significantly greater sEV number than control cells. sEVs were enriched in miR-21-5p and miR-217, which target DNMT1 and SIRT1. Treatment of control cells with SEN sEVs induced a miR-21/miR-217-related impairment of DNMT1-SIRT1 expression, the reduction of proliferation markers, the acquisition of a senescent phenotype and a partial demethylation of the locus encoding for miR-21. MicroRNA profiling of sEVs from plasma of healthy subjects aged 40-100 years showed an inverse U-shaped age-related trend for miR-21-5p, consistent with senescence-associated biomarker profiles. Our findings suggest that miR-21-5p/miR-217 carried by SEN sEVs spread pro-senescence signals, affecting DNA methylation and cell replication.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048230 | PMC |
| http://dx.doi.org/10.1080/20013078.2020.1725285 | DOI Listing |
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