Background And Aim: Infectious laryngotracheitis virus (ILTV) causes a highly pathogenic respiratory disease that affects poultry. It is also known as 1. ILT prophylaxis measures often include using live attenuated vaccines. The live attenuated vaccine can, however, lead to the formation of new strains of ILTV as a result of vaccine reversion and recombination with field strains. Therefore, this study was performed to explore the multilocus variation of ILTV strains of field and vaccine origin. Samples were tested from two distinctive geographical areas in Iraq as little is known about the ILTV genetic diversity within these areas.
Materials And Methods: The polymerase chain reaction method was utilized to generate sequencing templates of six highly polymorphic genes, including UL54, UL52, gB, ICP18.5, ICP4, and gJ in the layer chicken sample (n=15). The Western blotting technique was also employed to detect and estimate the native molecular weight of gE.
Results: The results revealed an important degree of genetic relatedness between the field and vaccine strains across all genes. In addition, gE was found to be expressed natively at 49 kDa.
Conclusion: The findings of this study may be used to improve the production process of the vaccine for more effective ILT prophylaxis and could further the understanding of epidemiologists and immunologists to better control ILT in the future.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020129 | PMC |
| http://dx.doi.org/10.14202/vetworld.2020.170-176 | DOI Listing |
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Article Synopsis
- - Multiple outbreaks of avian infectious laryngotracheitis (ILT) in chickens have been linked to widespread vaccination, with specific strains evolving toward the vaccine version, affecting the poultry industry economically despite biosecurity efforts.
- - Researchers isolated an ILTV strain from symptomatic chickens in China, which showed strong growth in eggs but not in liver cells, indicating differences in how the virus interacts with various types of cells.
- - The study identified a key genetic mutation in the virus and demonstrated its ability to cause disease in young chickens, enhancing knowledge about ILTV's biology and supporting future vaccine development efforts.
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