Aim: This study was aimed to investigate antimicrobial and cytotoxicity effect of nano ZnO in for the application of livestock feed supplement.
Materials And Methods: Nano ZnO was synthesized by wet chemical precipitation method using zinc acetate as a precursor and sodium hydroxide was used for reducing the precursor salt. The properties of synthesized powder were characterized using ultraviolet (UV)-visible spectroscopy, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD), respectively. antimicrobial activities were analyzed against the pathogenic bacteria in poultry , , , and . 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to analyze the cytotoxicity effect of nano ZnO.
Results: SEM showed a spherical ZnO particle in the range of 70-100 nm. The size of the particle and purity of the sample were confirmed by XRD. The nano-sized ZnO particles exhibited the UV absorption peak at 335 nm. In FTIR spectroscopy, pure ZnO nanoparticles showed stretching vibrations at 4000-5000 cm. ZnO nanoparticles exhibited remarkable antibacterial activity against , , , and bacterial strains. Cell viability was significantly reduced in a dose-dependent manner in the cytotoxicity study.
Conclusion: From the broad-spectrum antibacterial activity and the lower cytotoxicity observed at the prescribed dose, it is concluded that nano ZnO powder is a potential alternate zinc supplement for livestock.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020125 | PMC |
| http://dx.doi.org/10.14202/vetworld.2020.121-126 | DOI Listing |
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