Background And Aims: B cells involvement in animal models of atherosclerosis has been unequivocally established. However, the role of these cells in patients with atherosclerosis is almost unknown. Besides the production of antibodies, B cells can also exhibit regulatory functions mainly through IL-10. Here, we characterized human B cell subsets, their production of IL-10 in patients with atherosclerosis and their potential association with inflammation.
Methods: Patients with confirmed atherosclerotic events and controls with low cardiovascular risk were included. B cells subsets were determined in mononuclear cells (PBMC) using flow cytometry. PBMC were cultured (5 h) and (48 h) to determine IL-10 B cells and in some cases TNF-α and IFN-γ CD4 T cells. The inflammatory state of the participants was determined through high sensitivity C reactive protein levels.
Results: Increase in percentage and number of plasmablasts was observed in patients with atherosclerosis compared with controls. A decreased frequency of IL-10 B cells was observed in patients, both in and cultures. This decrease was detected in transitional, memory, and plasmablast subsets. Interestingly, the reduction of IL-10 B cells negatively and significantly correlated with the inflammatory condition of the studied subjects and associated with an increased frequency of TNF-α and IFN-γ CD4 T cells. The blockade of IL-10R did not show further effect in T cells activation.
Conclusions: There is an association between the inflammatory state and a reduction of IL-10 B cells that could contribute to the development of atherosclerosis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057201 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2020.e03441 | DOI Listing |
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