Colorimetric biosensors have been widely applied to mycotoxins testing. However, the colorimetric assay previously reported used a single color to detect one mycotoxin, and there were few reports on the simultaneous detection of multiple mycotoxins. In this work, a colorimetric biosensor for dual mycotoxins detection was developed. A FeO/GO based platform for aflatoxins B1 (AFB1) detection and a FeO@Au based platform for ochratoxin A (OTA) detection were fabricated. The quantification of OTA and AFB1 was respectively achieved by the release of thymolphthalein under alkaline conditions and 3,3',5,5'-tetramethylbenzidine was catalyzed by Au NPs under acidic conditions. Because of different conditions, two sensing methods didn't interfere with each other but could provide a higher detection efficiency. The detection range of AFB1 is 5-250 ng·ml and that of OTA is 0.5-80 ng·ml. This biosensor has been successfully applied in real sample detection, which has a broad application prospect in fields of food safety.
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| http://dx.doi.org/10.1016/j.foodchem.2020.126544 | DOI Listing |
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