Org Biomol Chem
UCL Chemistry Department, University College London, Gower Street, London, WC1E 6BT, UK.
Published: March 2020
Due to their homogeneity, tuneable properties, low cost and ease of manufacture, thermally induced phase separation (TIPS) polymeric microparticles are emerging as an exciting class of injectable device for the treatment of damaged tissue or complex diseases, such as cancer. However, relatively little work has explored enhancing surface functionalisation of this system. Herein, we present the functionalisation of TIPS microparticles with both small molecules and an antibody fragment of Herceptin™, via a heterobifunctional pyridazinedione linker capable of participating in SPAAC "click" chemistry, and compare it to the traditional method of preparing active-targeted microparticle systems, that is, physisorption of antibodies to the microparticle surface. Antigen-binding assays demonstrated that functionalisation of microparticles with Herceptin Fab, via a pyridazinedione linker, provided an enhanced avidity to HER2+ when compared to traditional physisorption methods.
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http://dx.doi.org/10.1039/d0ob00106f | DOI Listing |
Langmuir
January 2025
Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, United Kingdom.
Mass transfer governs the overall catalytic performance of heterogeneous catalysts considerably; however, this fundamental research has often been ignored. Here, macroporous SiO-supported Pt nanoparticle (Pt/SiO-M) and mesoporous SiO-supported Pt nanoparticle (Pt/SiO-m) catalysts were specifically fabricated by a facile thermal reduction step to engineer the resultant Pt nanoparticles showing similar physiochemical properties while possessing completely different porous microstructures exclusively originating from SiO supports. On this basis, a platform to explore the crucial mass transfer difference affecting catalytic activity is then established by systematically practicing industry-important benzene oxidation measurements.
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January 2025
Department of Materials Science and Engineering, Iowa State University, Ames, IA 50011.
From molecular dynamics (MD) simulations of melt-quenching and thermal aging procedures in pure Ag, Cu, Ag-Cu binary alloys, and Cu-Zr binary alloys, we have identified two distinct amorphous phases for a metastable undercooled liquid: the homogeneous L-phase with low shear rigidity and the heterogenous G-phase with much higher shear rigidity and a heterogeneity length scale Λ. Here, we examine two-phase equilibration studies showing that the G-phase melts to form the L-phase above ~1,000 K, which then transforms to form the crystal (X) phase; however, below the melting point of the G-Phase (~990 K), the X- and G-phases do not transform into each other. We suggest the presence of a G-phase is likely responsible for embrittlement often observed in metallic glasses.
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January 2025
College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetable Processing of Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing, China.
Unlabelled: The SpoVAF/FigP complex, a newly identified dormant spore ion channel, has been shown to amplify the response of germinant receptors (GRs) to nutrient germinants. However, its contribution to high-pressure-induced germination remains unexplored. In this study, we discovered that the 5AF/FigP complex played an important role in the GR-dependent germination of spores under moderate high pressure (MHP) by facilitating the release of ions, such as potassium (K), a mechanism in parallel with its role in nutrient-induced germination.
View Article and Find Full Text PDFFront Pharmacol
January 2025
IDiBE-Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche, Universidad Miguel Hernández, Elche, Spain.
The Selectivity Filter (SF) in tetrameric K channels, has a highly conserved sequence, TVGYG, at the extracellular entry to the channel pore region. There, the backbone carbonyl oxygens from the SF residues, create a stack of K binding sites where dehydrated K binds to induce a conductive conformation of the SF. This increases intersubunit interactions and confers a higher stability to the channel against thermal denaturation.
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January 2025
Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Inspired from heat shock proteins (HSPs), a thermo-sensitive coacervate-forming polycaprolactone (CPCL) was designed as a natural chaperone mimic to protect proteins from thermal stress. Unlike the coil-globule polymers of poly(-isopropyl acrylamide) (PNIPAM), the as-designed CPCL underwent a partial dehydration during heating, characterizing it as a coacervate-forming polymer. With its ability to transform between the coil and coacervate states in response to temperature, theCPCL spontaneously captured and released targeted proteins, thereby behaving like a natural chaperone of HSPs.
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