AI Article Synopsis
- The study investigates how adenosine A1 receptors in the hippocampus affect GSK-3 phosphorylation levels in mice undergoing cerebral ischemia-reperfusion injury, particularly with electroacupuncture pretreatment.
- Three groups were created: the electroacupuncture pretreatment group, the MCAO group (which underwent middle cerebral artery occlusion), and a sham-operated group, to compare neurobehavioral changes and cerebral infarction volume across groups.
- Results show that electroacupuncture pretreatment significantly reduced infarct size and improved neurological scores by increasing GSK-3 phosphorylation levels through the activation of A1 receptors, suggesting a protective effect against ischemia-reperfusion injury.
Article Abstract
Objective: To observe the effect of adenosine A1 receptor in the hippocampus of mice on GSK-3 phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Α1 receptor mediating cerebral ischemia-reperfusion injury.
Method: The model of middle cerebral artery occlusion (MCAO) was established and grouped into electroacupuncture pretreatment group (EA group), MCAO group, and sham-operated group (Sham group). The neurobehavioral manifestation, the volume of cerebral infarction, and its related protein changes in mice in each group were observed. Then, adenosine Α1 receptor antagonist and agonist were injected intraperitoneally to observe the effects of A1 receptor on the phosphorylation level of GSK-3 phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Α1 receptor mediating cerebral ischemia-reperfusion injury.
Results: (1) Compared with the MCAO group (24 hours after reperfusion), the infarct size in the EA group decreased significantly, and the Garcia neurological score and phosphorylation level of GSK-3 phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Α1 receptor mediating cerebral ischemia-reperfusion injury. phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Α1 receptor mediating cerebral ischemia-reperfusion injury. phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Α1 receptor mediating cerebral ischemia-reperfusion injury.
Conclusions: Electroacupuncture pretreatment can increase GSK-3 phosphorylation level via activating A1 receptor, to protect neurons in ischemia-reperfusion injury. phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Α1 receptor mediating cerebral ischemia-reperfusion injury.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054798 | PMC |
| http://dx.doi.org/10.1155/2020/6848450 | DOI Listing |
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