AI Article Synopsis
- The outbreak of unexplained pneumonia in Wuhan in December 2019 led to the identification of the coronavirus (SARS-CoV-2) as the cause, resulting in the naming of the disease COVID-19 by WHO.
- Samples from suspected COVID-19 patients in South Korea were tested, and methods like cell inoculation and genome sequencing confirmed the presence of the virus.
- The isolated virus, with over 99.9% genetic similarity to previously identified SARS-CoV-2 strains from other countries, was named BetaCoV/Korea/KCDC03/2020, demonstrating distinctive SARS-CoV-like features through electron microscopy.
Article Abstract
Objectives: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
Methods: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
Results: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
Conclusion: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880 | PMC |
| http://dx.doi.org/10.24171/j.phrp.2020.11.1.02 | DOI Listing |
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