AI Article Synopsis
- The study shows that neurons from mouse embryos can survive in adult brains and connect to the spinal cord, hinting at cell transplantation as a potential method to repair the corticospinal tract (CST).
- Researchers discovered that grafted cells in the adult brain expressed a protein called CTIP2, which is related to axon growth in the CST.
- By isolating L1CAM+ cells, they found these cells were more effective in generating axons compared to other cells, suggesting that sorting these specific cells could enhance efforts to reconstruct the CST.
Article Abstract
The cerebral cortical tissue of murine embryo and pluripotent stem cell-derived neurons can survive in the adult brain and extend axons to the spinal cord. These features suggest that cell transplantation can be a strategy to reconstruct the corticospinal tract (CST). It is unknown, however, which cell population makes for safe and effective donor cells. To address this issue, we grafted the cerebral cortex of E14.5 mouse to the brain of adult mice and found that the cells in the graft extending axons along the CST expressed CTIP2. By using CTIP2:GFP knock-in mouse embryonic stem cells (mESCs), we identified L1CAM as a cell surface marker to enrich CTIP2 cells. We sorted L1CAM cells from E14.5 mouse brain and confirmed that they extended a larger number of axons along the CST compared to L1CAM cells. Our results suggest that sorting L1CAM cells from the embryonic cerebral cortex enriches subcortical projection neurons to reconstruct the CST.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042175 | PMC |
| http://dx.doi.org/10.3389/fncel.2020.00031 | DOI Listing |
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