Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO-EtOH-HO fluids (average molar composition, Χ 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO-limonene fluids (Χ 0.88) and neat supercritical CO (scCO) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO-limonene followed by enzyme treatment, resulted in efficient DNA removal. The structure of elastic fibers was preserved after pressurized treatment, regardless solvent composition. In conclusion, pressurized CO-ethanol fluids offer an efficient tool for delipidation in bio-scaffold production, while pressurized CO-limonene fluids facilitate subsequent enzymatic removal of DNA.
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http://dx.doi.org/10.1038/s41598-020-60827-4 | DOI Listing |
J Environ Manage
December 2024
Nuclear and Engineering Nonproliferation Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.
Monitoring nuclear reactor operations is vital for nuclear safeguards as it ensures that reactors are in compliance with international legal agreements. Validating nuclear facilities and activities, including potential clandestine activities, is currently accomplished by using remotely sensed data from satellites and aircrafts and on-site sampling. However, these techniques are temporally-limited as sampling and interpretation of environmental releases frequently involve labor-intensive, on-site collections.
View Article and Find Full Text PDFGut Microbes
December 2025
Hypertension Research Laboratory, School of Biological Sciences, Faculty of Science, Monash, Clayton, Australia.
The gut microbiota is a crucial link between diet and cardiovascular disease (CVD). Using fecal metaproteomics, a method that concurrently captures human gut and microbiome proteins, we determined the crosstalk between gut microbiome, diet, gut health, and CVD. Traditional CVD risk factors (age, BMI, sex, blood pressure) explained < 10% of the proteome variance.
View Article and Find Full Text PDFKnee Surg Sports Traumatol Arthrosc
December 2024
Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Purpose: To explore if recentering the humeral head could improve shoulder abduction in shoulders with irreparable massive rotator cuff tears (IMRCTs).
Methods: Nine fresh-frozen cadaveric shoulders were used to simulate the intact condition and IMRCTs as controls. Four 'recenter' interventions were then sequentially applied: subacromial interposition (SI) using 4- and 8-mm spacers and superior capsule reconstruction (SCR) using two- and four-layer fascia lata grafts.
Antimicrob Resist Infect Control
December 2024
Infection Control Team, Queen Mary Hospital, Hong Kong West Cluster, Pokfulam, Hong Kong Special Administrative Region, China.
Background: Hand hygiene is a critical component of infection prevention in healthcare settings. Innovative strategies are required to enhance hand hygiene practices among patients and healthcare workers (HCWs).
Methods: This study was conducted at the Chemotherapy Day Center of Queen Mary Hospital, Hong Kong.
Gut Pathog
December 2024
Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Background: Maintaining gut microbial homeostasis is crucial for human health, as imbalances in the gut microbiota (GM) can lead to various diseases, including metabolic syndrome (MS), exacerbated by the use of antipsychotic medications such as olanzapine (OLZ). Understanding the role of the GM in OLZ-induced MS could lead to new therapeutic strategies. This study used metagenomic analysis to explore the impact of OLZ on the GM composition and examined how probiotics can mitigate its adverse effects in a rat model.
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