sp. nov., a multiple-stress-tolerant bacterium isolated from a deep-sea sediment sample of the New Britain Trench.

A piezotolerant, HO-tolerant, heavy-metal-tolerant, slightly halophilic bacterium (strain NBT06E8) was isolated from a deep-sea sediment sample collected from the New Britain Trench at depth of 8900 m. The strain was aerobic, motile, Gram-stain-negative, rod-shaped, oxidase-positive and catalase-positive. Growth of the strain was observed at 4-45 °C (optimum, 30 °C), at pH 5-11 (optimum, pH 8-9) and in 0.5-21 % (w/v) NaCl (optimum, 3-7 %). The optimum pressure for growth was 0.1-30 MPa with tolerance up to 60 MPa. Under optimum growth conditions, the strain could tolerate 15 mM HO. Resuls of 16S rRNA gene sequence analysis showed that strain NBT06E8 is closely related to DSM 30161 (99.5%), DSM 5425 (99.43%) and Althf1 (99.35%). The digital DNA-DNA hybridization values between strain NBT06E8 and the three related type strains, , and , were 30.5±2.4 %, 30.7±2.5% and 31.5±2.5 %, respectively. The average nucleotide identity values between strain NBT06E8 and the three related type strains were 86.26, 86.26 and 83.63 %, respectively. The major fatty acids were summed feature 8 (C ω7 and/or C ω6) and C. The predominant respiratory quinone detected was ubiquinone-9 (Q-9). Based on its phenotypic and phylogenetic characteristics, we conclude that strain NBT06E8 represents a novel species of the genus , for which the name sp. nov. is proposed (type strain NBT06E8= MCCC 1K04228=KCTC 72680).