Vitrification is a superior method for cryopreservation of IVF embryos, but due to complicated warming protocols, it is not commonly used for commercial bovine embryos routine. To overcome the need of laboratory embryo preparation during warming, we developed an in-straw warming protocol compatible with most vitrification devices for embryo transfer without sucrose gradient steps and embryo evaluation. Surprisingly, one of the tested protocols improved embryo survival (95.0%* vs 83.1% expansion rate and 74.2%* vs 51.5% hatching rate) compared to conventional in-plate warming. Embryo quality was also increased, taken by the higher total cell numbers (160.7 ± 8.6* vs 99.0 ± 7.9) and lower apoptosis index (4.9 ± 0.6* vs 11.5 ± 2.4) 48 h after warming. Pregnancy rates were similar between vitrified-warmed embryos and fresh embryos (40% vs 43%). Based on our results, we suggest in-straw warming should always be used for vitrified embryos due to beneficial effects. Direct transfer can be safely performed using this protocol.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cryobiol.2020.02.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses.
Front Plant Sci
October 2024
State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
As the largest organic carbon input in the agroecosystems, crop residues can increase soil carbon sequestration and crop production in greenhouse vegetable fields (GVFs). However, the soil microbiological mechanisms driving straw decomposition in GVFs under different incubation temperatures and fertilization treatments are not clear. Thus, soil samples were collected from a long-term field experiment included chemical fertilizer application alone (CF), 2/4 fertilizer N+2/4 organic fertilizer N (CM), 2/4 fertilizer N+1/4 organic fertilizer N+1/4 straw N (CMS), 2/4 fertilizer N+2/4 straw N (CS), and incubated with C-labeled straw at different temperatures (15, 25, and 35°C) for 60 days.
View Article and Find Full Text PDFMethodological approaches in vitrification: Enhancing viability of bovine oocytes and in vitro-produced embryos.
Reprod Domest Anim
October 2024
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
Cryopreservation of bovine oocytes and embryos is essential for long-term preservation and widespread distribution of genetic material, particularly in bovine in vitro embryo production, which has witnessed substantial growth in the past decade due to advancements in reproductive biotechnologies. Among current cryopreservation methods, vitrification has emerged as the preferred cryopreservation technique over slow freezing for preserving oocytes and in vitro-produced (IVP) embryos, as it effectively addresses membrane chilling injury and ice crystal formation. Nonetheless, challenges remain and a simple and robust vitrification protocol that guarantees the efficiency and viability after warming has not yet been developed.
View Article and Find Full Text PDFBiochar carbon sequestration potential rectification in soils: Synthesis effects of biochar on soil CO, CH and NO emissions.
Sci Total Environ
December 2023
College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311215, China. Electronic address:
Biochar production and its soil sequestration are promising ways to mitigate global warming. Effects of biochar on soil CO, CH and NO release have been studied extensively. In contrast, few studies have comprehensively quantified and synthesized the effect of biochar on soil greenhouse gas (GHG) emission and coupled it to the calculation of carbon sequestration potential.
View Article and Find Full Text PDF[Effects of Aeration on Surface Water Nutrient Dynamics and Greenhouse Gas Emission in Different Straw Returning Paddy Fields].
Huan Jing Ke Xue
April 2023
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Straw returning is of great significance for improving soil structure, soil fertility, crop yield, and quality. However, straw returning causes environmental problems such as increased methane emission and non-point source pollutant emission risk. How to reduce the negative effects of straw returning is an urgent problem to be solved.
View Article and Find Full Text PDFVitrification and in-straw warming do not affect pregnancy rates of biopsied bovine embryos.
Theriogenology
October 2022
Department of Animal Medicine and Surgery, Autonomous University of Barcelona, Travessera dels Turons, s/n, ES-08193, Cerdanyola del Vallès Barcelona, Spain. Electronic address:
In the cattle industry, in vivo or in vitro embryo production combined with genotyping and cryopreservation technologies allows the selection and conservation of embryos carrying genes for desirable traits. This study aimed to assess the efficiency of a vitrification method suitable for in-straw warming of biopsied in vivo derived (IVD) bovine embryos. Three experiments were carried out using two methodologies: the Cryotop®, the gold standard vitrification and 3-step warming methodology, or the VitTrans, a vitrification/in-straw 1-step warming method that enables direct embryo transfer to the uterus.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!