Carbon monoxide‑releasing molecule‑3 (CORM‑3), which is an exogenous carbon monoxide (CO) compound, slowly releases CO under physiological conditions; this exerts neuroprotective effects against incomplete ischemia/reperfusion injury. The objective of the present study was to investigate whether the administration of CORM‑3 protects against nucleotide‑binding oligomerization domain‑like receptor pyrin domain‑3 (NLRP3) inflammasome formation and neuronal pyroptosis in the hippocampus following hemorrhagic shock and resuscitation (HSR). To establish this, an HSR model was created. Hemorrhagic shock was induced in adult male Sprague‑Dawley rats under sevoflurane anesthesia by bleeding using a heparinized syringe to maintain a mean arterial pressure of 30±5 mmHg for 60 min. Resuscitation was performed by reperfusion of the blood and, if necessary, administering sterile saline to achieve the baseline arterial pressure. Following resuscitation, CORM‑3 (4 mg/kg) was injected via the femoral vein. Neuronal pyroptosis in the hippocampus, mitochondrial morphology, mitochondrial DNA (mtDNA), brain magnetic resonance imaging, expression levels of NLRP3 and the interaction of pro‑caspase‑1 and apoptosis‑associated speck‑like protein containing a CARD domain (ASC) were examined 12 h after HSR; locomotor activity was assessed 7 days after HSR. Compared with HSR‑treated rats, CORM‑3 administration resulted in a lower level of neuronal pyroptosis in the hippocampus, improved mitochondrial morphology, a lower mtDNA level, steadier levels of metabolites, decreased expression levels of NLRP3 and pro‑caspase‑1 interacting with ASC and enhanced locomotor activity. In conclusion, treatment with CORM‑3 ameliorated impairments of locomotor and exploratory activities in a rat model of HSR. The mechanism may be associated with the inhibition of mitochondrial DNA‑induced pyroptosis via improvements in cell metabolism.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053849PMC
http://dx.doi.org/10.3892/ijmm.2020.4493DOI Listing

Publication Analysis

Top Keywords

hemorrhagic shock
12
neuronal pyroptosis
12
pyroptosis hippocampus
12
exogenous carbon
8
carbon monoxide
8
mitochondrial dna‑induced
8
arterial pressure
8
mitochondrial morphology
8
expression levels
8
levels nlrp3
8

Similar Publications

The Black Esophagus: A Rare Case Presentation.

Cureus

December 2024

Gastroenterology and Hepatology, Saint Peter's University Hospital, New Brunswick, USA.

Acute esophageal necrosis (AEN), also known as black esophagus or Gurvits syndrome, is an uncommon endoscopic finding characterized by diffuse, circumferential, black discoloration of the esophagus that terminates at the gastroesophageal junction. The incidence of AEN has been reported to be 0-0.2% in autopsy series and up to 0.

View Article and Find Full Text PDF

Patients with an unruptured ovarian pregnancy may exhibit signs of shock. Laparoscopy is well-suited for the detection of ovarian pregnancy. Viable pregnancies are possible for patients with ovarian pregnancy and a low risk of ectopic pregnancy.

View Article and Find Full Text PDF

Hemorrhagic shock is a significant cause of trauma-related mortality. Resuscitative endovascular balloon occlusion of the aorta (REBOA) is a less-invasive aortic occlusion maneuver for severe hemorrhagic shock but potentially inducing oxidative stress injuries. In an animal model, this study investigated hydrogen gas inhalation therapy's potential to mitigate post-REBOA ischemia-reperfusion injuries (IRIs).

View Article and Find Full Text PDF

Management of cardiac trauma and penetrating cardiac injuries with severe hemorrhagic shock: a 5-year experience.

J Trauma Inj

December 2024

Department of Cardiovascular and Thoracic Surgery, Tam Anh Hospital, Ho Chi Minh City, Vietnam.

Purpose: The diagnosis and management of cardiac trauma and penetrating cardiac injuries pose significant challenges in emergency settings due to the rapid onset of life-threatening complications. This paper presents a narrative review to better describe the etiology, presentation, and management of penetrating cardiac trauma, offering insights and experiences related to performing emergency surgery for such cases.

Methods: We compiled cases of traumatic cardiac rupture and penetrating cardiac injuries accompanied by severe hemorrhagic shock that necessitated emergency surgery.

View Article and Find Full Text PDF

Spontaneous liver bleeding is a rare but life-threatening complication of hepatocellular carcinoma (HCC). The optimal management strategy for this condition remains a topic of ongoing debate. We present the case of a 74-year-old man with cirrhosis and hemorrhagic shock resulting from the spontaneous rupture of HCC.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!