Capture and Detection of Lactate Dehydrogenase in a Bead-Based Multiplex Immunoassay.

Laboratory detection of malaria antigens has proved valuable for research and epidemiological purposes. We recently developed a bead-based multiplex antigen assay for pan- and targets. Here, we report integration of a -specific target to this multiplex panel: lactate dehydrogenase (PvLDH). Within the multiplex panel, assay signal for purified PvLDH antigen titrated into the single-digit picogram range. Against a panel of polymerase chain reaction (PCR)-confirmed samples from acute infections ( = 36), sensitivity was 91.7% in using PvLDH detection for identifying the presence of parasites. Specificity against a panel of persons with no infection ( = 44) was 100%, and specificity against a panel of PCR-confirmed or infections ( = 164) was 90.2%. Addition of this PvLDH capture and detection system into the multiplex antigen panel will now allow for sensitive screening for species identification of both and in the laboratory.