Phenotypic Investigation of the Antimicrobial Effect of Organic and Hydro-Alcoholic Extracts of on Oral Microbiota.

Objectives: Considering the emergence of resistant microbes and side effects of chemical drugs, in this study, the inhibitory effect of organic and hydro-alcoholic extracts of () on some oral microbiota was investigated.

Materials And Methods: In this experimental study, standard strains of (; PTCC 5027), (; PTCC 5295), (; PTCC 5297), and (; PTCC 1688) were collected from the Iranian Research Organization for Science and Technology (IROST). Then, the minimum inhibitory concentration (MIC) of organic and hydro-alcoholic extracts of was determined based on the CLSI protocol and using the micro-dilution method. The contents of each well were subcultured in Müller-Hinton agar ( species) and blood agar (). The lowest concentration with no growth was considered as the minimum fungicidal concentration (MFC) or bactericidal concentration (MBC). Statistical analyses were performed using Mann-Whitney test.

Results: Hydro-alcoholic extract of at the concentration of 50 mg/ml inhibited the growth of and . It also inhibited the growth of and at the concentration of 100 mg/ml. Organic extract of at the concentration of 200 mg/ml only inhibited the growth of .

Conclusion: Hydro-alcoholic extract of had a greater inhibitory effect on and compared to the organic extract.