Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission of malaria parasites by mosquitoes. CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology of vector-pathogen interactions and can itself form the basis of mosquito control strategies. However, embryo injection methods used to genetically manipulate mosquitoes (especially ) are difficult and inefficient, particularly for non-specialist laboratories. Here, we adapted the ReMOT Control (ceptor-ediated vary ransduction of argo) technique to deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector without injecting embryos. In , ReMOT Control gene editing was as efficient as standard embryo injections. The application of ReMOT Control to opens the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise to perform embryo injections and establishes the flexibility of ReMOT Control for diverse mosquito species.
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| http://dx.doi.org/10.1534/g3.120.401133 | DOI Listing |
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