Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant with Lipoxygenase-1 and Hydroperoxide Lyase Genes from .

is an ectomycorrhizal fungus, related with the host of . Most of studies on have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of using the yeast as a host. In this study, cDNA genes correlated with these reactions were cloned from , and expression studies of theses genes were carried out in the yeast . The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of in recombinant was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with represents a significant step forward.