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http://dx.doi.org/10.1093/nar/16.24.11842 | DOI Listing |
Sci Rep
January 2025
Animal Health, ICAR-National Research Centre on Pig, Rani, Guiwahati, 781131, Assam, India.
This research aimed to characterize the mitochondrial genome of the Ghoongroo (GH) pig, a notable breed in India, along with its crossbred varieties, to elucidate their matrilineal components, evolutionary history, and implications for conservation. Seven pigs (5 GH, 2 crossbred, namely Rani and Asha) were sequenced for complete mitochondrial genome, while 24 pigs (11 GH, 6 Rani, and 7 Asha) were sequenced for the complete D-loop of the mitochondrial genome. The genome size of these pigs was determined to be 16,690 bp.
View Article and Find Full Text PDFParasit Vectors
January 2025
Veterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Background: Brazilian spotted fever is a tick-borne disease caused by the bacterium Rickettsia rickettsii, whose main vector in Brazil is the tick Amblyomma sculptum. Amplifying hosts are essential for the perpetuation of this bacterium in the tick population as they can be sources of infection during bacteremic periods. Recent studies demonstrated the ability of suids (Sus scrofa) to sustain populations of A.
View Article and Find Full Text PDFJ Arrhythm
February 2025
Department of Cardiology Institute of Medicine, University of Tsukuba Tsukuba Japan.
Background/objectives: Very high-power and short-duration (vHPSD) ablation with QDOT MICRO™ facilitates speedy and safe ablation for pulmonary vein isolation. A brief time interval between ablating two neighboring sites with vHPSD may potentially influence the size and geometry of the lesions. This study evaluates lesion formation when delivering adjacent applications using vHPSD at various inter-lesion times (ILTs).
View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2025
ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Two Gram-stain-negative cocci anaerobes were isolated from pig faeces and designated as strains YH-vei2232 and YH-vei2233. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to KCTC 5967, with 97.0% similarity.
View Article and Find Full Text PDFiScience
January 2025
Laboratory of Antibody Discovery and Accelerated Protein Therapeutics, Center for Infectious Diseases, Houston Methodist Research Institute and Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, TX, USA.
T7 RNA polymerase (RNAP) has enabled orthogonal control of gene expression and recombinant protein production across diverse prokaryotic host chassis organisms for decades. However, the absence of 5' methyl guanosine caps on T7 RNAP-derived transcripts has severely limited its utility and widespread adoption in eukaryotic systems. To address this shortcoming, we evolved a fusion enzyme combining T7 RNAP with the single subunit capping enzyme from African swine fever virus using .
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