AI Article Synopsis
- Visceral leishmaniasis (VL) is a significant neglected tropical disease with diagnostic challenges, but loop-mediated isothermal amplification (LAMP) shows promise as a point-of-care testing tool for VL.
- A systematic review was conducted analyzing LAMP's effectiveness for diagnosing VL between 2000 and 2019, using multiple academic databases for comprehensive research.
- The study suggests LAMP could serve as a preliminary screening method, reducing unnecessary tissue aspirations, and calls for further research and collaboration among institutions in VL-affected regions.
Article Abstract
: Visceral leishmaniasis (VL) is a life-threatening infection remaining as one of the most neglected tropical diseases around the world. Despite scientific advances, an accurate diagnosis of VL remains a challenge. Loop-mediated isothermal amplification (LAMP) has emerged as a promising diagnostic tool with the possibility of becoming a point-of-care test to guide VL diagnosis and treatment.: We conducted a systematic review assessing LAMP systems for diagnosing VL from 2000 to 2019. We performed structured searches in PubMed, LILACS, Scopus, and Web of Science without language restriction. Two reviewers screened articles, completed the data extraction and assessment of the risk of bias. A qualitative summary of the included studies was performed.: LAMP could be used as a screening test for VL diagnosis, so tissue aspiration could be performed only for those who are LAMP negative. We recommend more studies about the performance of the Loopamp™ Detection kit and the Brazilian LAMP assay. Thus, we expect in the future the constitution of an international consortium to share experiences, projects, and other LAMP approaches mainly among researchers and institutions located within VL endemic countries.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/14737159.2020.1736564 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Legionella Pneumonia Undetected by Repeated Urinary Antigen Testing With Ribotest® Legionella.
Cureus
November 2024
Department of Clinical Infectious Diseases, Tokoname City Hospital, Tokoname, JPN.
This report presents a patient with pneumonia (LP), initially presented with fever and mild hypoxemia, with subsequent progression to severe pneumonia during hospitalization. Despite multiple negative urinary antigen tests using Ribotest , the diagnosis was confirmed via the loop-mediated isothermal amplification method of lower respiratory tract secretions. This case highlights the diagnostic limitations of Ribotest and emphasizes the importance of a comprehensive diagnostic strategy, incorporating nucleic acid amplification tests or culture in suspected patients with LP.
View Article and Find Full Text PDFAn integrated and paper-based microfluidic system employing LAMP-CRISPR and equipped with a portable device for simultaneous detection of pathogens.
Anal Bioanal Chem
December 2024
School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, PR China.
Point-of-care testing methods are essential for the large-scale diagnosis and monitoring of bacterial infections. This study introduces an integrated platform designed for the simultaneous detection of pathogenic bacteria. Users can simply inject samples into the system, which then conducts the entire procedure in a fully automated manner, eliminating the need for external power sources, all within 60 min or less.
View Article and Find Full Text PDFDevelopment of a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Beiji nairovirus.
Microb Pathog
December 2024
School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong Province, China; Department of Infectious Diseases, Center for Pathogen Biology and Infectious Diseases, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun 130122, Jilin Province, China; Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, Guangdong Province, China. Electronic address:
Beiji nairovirus (BJNV) is a recently discovered tick-borne RNA virus associated with human febrile illness. This study aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the precise detection of BJNV, with a specific focus on assessing its effectiveness with clinical samples. The optimal molecular target was identified as the BJNV small (S) segment gene, and the ideal reaction conditions were established at 65 °C for 50 min.
View Article and Find Full Text PDFA Case of Refractory Kawasaki Disease With Yersinia pseudotuberculosis Infection Successfully Treated With Cefotaxime Following Immunosuppressive Therapy.
Cureus
November 2024
Department of Pediatrics, Japanese Red Cross Wakayama Medical Center, Wakayama, JPN.
Kawasaki disease (KD) is a vasculitis mainly affecting children under five, with symptoms such as persistent fever, rash, red lips, strawberry tongue, conjunctivitis, and swollen hands and feet. Diagnosis is based on a fever lasting over five days plus at least four of these symptoms. Treatment includes intravenous immunoglobulin (IVIG) and aspirin to reduce complications, especially coronary artery issues.
View Article and Find Full Text PDFIntegrating spore trapping technology with loop-mediated isothermal amplification assay for surveillance and sustainable management of rice false smut disease.
Front Microbiol
December 2024
Agro Climate Research Centre (ACRC), Tamil Nadu Agricultural University, Coimbatore, India.
Rice ( L.) is a vital crop feeding more than half of the world's population, with production occurring predominantly in Asian countries. However, rice cultivation faces challenges from various fronts, including biotic stresses intensified by climate change.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!