It is common sense that chewing a mint leaf can cause a cooling feeling, while chewing ginger root will produce a burning feeling. In Traditional Chinese Medicine (TCM), this phenomenon is referred to as 'cold/hot' properties of herbs. Herein, it is reported that TCM with different "cold/hot" properties have different effects on the variation of cells. To explore the intrinsic 'cold/hot' properties of TCM from the perspective of cellular and molecular biology. A375 cells were selected using Cancer Cell Line Encyclopaedia (CCLE) analysis and western blots. Hypaconitine and baicalin were selected by structural similarity analysis from 56 and 140 compounds, respectively. A wireless thermometry system was used to measure cellular temperature change induced by different compounds. Alteration of intracellular calcium influx was investigated by means of calcium imaging. The IC values of GSK1016790A, HC067047, hypaconitine, and baicalin for A375 cells are 8.363 nM, 816.4 μM, 286.4 μM and 29.84 μM, respectively. And, 8 μM hypaconitine induced obvious calcium influx while 8 μM baicalin inhibited calcium influx induced by TRPV4 activation. Cellular temperature elevated significantly when treated with GSK1016790A or hypaconitine, while the results were reversed when cells were treated with HC067047 or baicalin. The changes in cellular temperature are speculated to be caused by the alteration of intracellular calcium influx mediated by TRPV4. In addition, the 'cold/hot' properties of compounds in TCM can be classified by using cellular temperature detection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067177 | PMC |
| http://dx.doi.org/10.1080/13880209.2020.1732429 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Immunomodulatory properties of polysaccharide extract samples from Cyanobacterium sp. Rippka B-1200.
Sci Rep
December 2024
Sec "Applied Biotechnologies", Immanuel Kant Baltic Federal University, Kaliningrad, Russia, 236040.
Cyanobacteria are most abundant in aquatic systems and can grow in freshwater, saline or brackish water, and cold/hot springs. Cyanobacteria have attracted considerable research attention in the last decade as a potential source of numerous biological products in large quantities, such as biofuels, pigments, polyunsaturated fatty acids, nutraceuticals, enzymes, and polysaccharides. Unlike most plant and fungal polysaccharides, the chemical composition, immunomodulatory activity, and molecular mechanisms of action of Cyanobacterium sp.
View Article and Find Full Text PDFMicrostructural evolution of bio-based chitosan aerogels for thermal insulator with superior moisture/fatigue resistance and anti-thermal-shock.
Int J Biol Macromol
October 2024
Polymer Aerogels Research Center, Jiangxi University of Science and Technology, Nanchang 330013, Jiangxi, China; Thermal Control Technology Laboratory of Aircraft in Space Environment, Jiangxi University of Science and Technology, Nanchang 330013, Jiangxi, China. Electronic address:
Bio-based aerogel is a functionalized nanoporous material with environmentally friendly, high surface area, ultra-low density, high porosity, and low thermal conductivity, making it suitable for various applications such as energy-saving buildings, electronic information, separation, adsorption, catalysis, biomedicine, and others. However, the current bio-based chitosan aerogel still faces great challenges in reaching multifunctional improvement to address its intrinsic shortcomings. Herein, we propose a new approach depending upon supramolecular interactions for constructing chitosan/bacterial cellulose aerogels that simultaneously possess superior moisture resistance/fatigue, anti-thermal-shock, and flame retardancy.
View Article and Find Full Text PDFStudy on the fourof Pfaffia glomerata based on the metabolomics technology and comparison of Dangshen () in the equivalent substitution prescription.
J Tradit Chin Med
August 2024
National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences (CACMS), Beijing 100700, China.
Objective: To explore the four of Pfaffia glomerata (PG) and endow this foreign folk herb with the properties of Chinese medicine, make it Chinese medicinal and localized, and could be used as a Chinese medicine.
Methods: The normal group, six cold herb groups, six hot herb groups, six cool herb groups, PG prescription group (PGPG), and Dangshen () prescription group (CPPG) were prepared with corresponding concentrations of water extracts, these herb extracts were administered by gavage to Sprague-Dawley rats, and the 12 h urine at night on the 29th day of the SD rats in each group were collected, Liquid Chromatograph Mass Spectrometer system was used to analyze them, the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up, so as to the medicinal properties of PGPG, CPPG and PG were predicted. Based on the Progenesis QI, Human Metabolome Database, Kyoto Encyclopedia of Genes and Genomes, MetaboAnalyst 5.
Temperature-resilient and sustainable Mn-MOF oxidase-like nanozyme (UoZ-4) for total antioxidant capacity sensing in some citrus fruits: Breaking the temperature barrier.
Food Chem
August 2024
Department of Chemistry, College of Science, University of Sulaimani, Qliasan St., 46002 Sulaymaniyah, Kurdistan region, Iraq.. Electronic address:
Current nanozyme applications rely heavily on peroxidase-like nanozymes and are limited to a specific temperature range, despite notable advancements in nanozyme development. In this work, we designed novel Mn-based metal organic frameworks (UoZ-4), with excellent oxidase mimic activity towards common substrates. UoZ-4 showed excellent oxidase-like activity (with Km 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!