Murine norovirus (MNV), is a prevalent pathogen of laboratory mice closely related to human norovirus (HuNoV), a contagious pathogen known to cause gastroenteritis worldwide; however, the mechanism of norovirus replication remains poorly understood. Both heat shock protein 90 (Hsp90) and heat shock protein 70 (Hsp70) play an important role in viral genome replication and viral gene expression. In this study, we first found that heat stress exerted a positive effect on the replication of MNV in the murine macrophage RAW264.7 cell line. Inhibition of Hsp70 and Hsp90 by the specific inhibitors, KNK437 and 17-AGG, respectively showed that Hsp70 and Hsp90 enhanced MNV genome replication and virion production. In addition, we found that KNK437 and 17-AGG could decrease the level of IL-1β, IL-10, and TNF-α mRNA expression in MNV-infected cells. These data suggested that heat stress can positively regulate MNV replication, which advances our understanding of the molecular mechanism of MNV infection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.micpath.2020.104102 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impact of exogenous rhamnolipids on plant photosynthesis and biochemical parameters under prolonged heat stress.
Photosynthetica
January 2025
University of Reims Champagne-Ardenne, INRAE, RIBP, USC 1488, 51100 Reims, France.
High temperatures severely affect plant growth and development leading to major yield losses. These temperatures are expected to increase further due to global warming, with longer and more frequent heat waves. Rhamnolipids (RLs) are known to protect several plants against various pathogens.
View Article and Find Full Text PDFFlow Cytometry Analysis of Perturbations in the Bacterial Cell Envelope Enabled by Monitoring Generalized Polarization of the Solvatochromic Peptide UNR-1.
Anal Chem
January 2025
Laboratoire d'Innovation Thérapeutique, UMR7200 CNRS, Université de Strasbourg, Institut du Médicament de Strasbourg, 74 route du Rhin, Strasbourg F-67000, France.
The worldwide spread of antibiotic resistance is considered to be one of the major health threats to society. While developing new antibiotics is crucial, there is also a strong need for next-generation analytical methods for studying the physiological state of live bacteria in heterogeneous populations and their response to environmental stress. Here we report a single-cell high-throughput method to monitor changes in the bacterial cell envelope in response to stress based on ratiometric flow cytometry.
View Article and Find Full Text PDFProtein palmitoylation is involved in regulating mouse sperm motility via the signals of calcium, protein tyrosine phosphorylation and reactive oxygen species.
Biol Res
January 2025
School of Pharmacy, Hangzhou Medical College, Hangzhou, Zhejiang, China.
Background: Protein palmitoylation, a critical posttranslational modification, plays an indispensable role in various cellular processes, including the regulation of protein stability, mediation of membrane fusion, facilitation of intracellular protein trafficking, and participation in cellular signaling pathways. It is also implicated in the pathogenesis of diseases, such as cancer, neurological disorders, inflammation, metabolic disorders, infections, and neurodegenerative diseases. However, its regulatory effects on sperm physiology, particularly motility, remain unclear.
View Article and Find Full Text PDFSlight thermal stress exerts genetic diversity selection at coral (Acropora digitifera) larval stages.
BMC Genomics
January 2025
Sesoko Marine Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu, Okinawa, 905-0227, Japan.
Background: Rising seawater temperatures increasingly threaten coral reefs. The ability of coral larvae to withstand heat is crucial for maintaining reef ecosystems. Although several studies have investigated coral larvae's genetic responses to thermal stress, most relied on pooled sample sequencing, which provides population-level insights but may mask individual genotype variability.
View Article and Find Full Text PDFInterplay of p23 with FKBP51 and their chaperone complex in regulating tau aggregation.
Nat Commun
January 2025
Department for NMR-based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
The pathological deposition of tau and amyloid-beta into insoluble amyloid fibrils are pathological hallmarks of Alzheimer's disease. Molecular chaperones are important cellular factors contributing to the regulation of tau misfolding and aggregation. Here we reveal an Hsp90-independent mechanism by which the co-chaperone p23 as well as a molecular complex formed by two co-chaperones, p23 and FKBP51, modulates tau aggregation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!