AI Article Synopsis
- A set of polymorphic nuclear microsatellite loci was developed to study population genetics in Agavaceae and related species.
- Researchers extracted sequences from genome data and tested the polymorphism of 19 loci across four populations in Central and Eastern Europe, finding varying levels of alleles and heterozygosity.
- The new microsatellite markers were shown to work well in closely related species, making them valuable for future genetic studies.
Article Abstract
Premise: A set of polymorphic nuclear microsatellite loci was developed and tested for use in population genetic analyses of (Agavaceae) and related species.
Methods And Results: Sequences of 110 primers were extracted in silico from Illumina MiSeq genome skimming data. The degree of polymorphism of 19 loci was tested in four . populations collected in Central and Eastern Europe. The average number of alleles per loci ranged from two to 17, and levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.100 to 0.900, respectively. Primers were successfully amplified in the closely related species (12 loci) and (six loci), whereas they mostly failed to amplify in the phylogenetically more-distant species (three loci) and . (no amplification).
Conclusions: This newly developed set of polymorphic nuclear microsatellite markers will be useful for population genetic investigation of . and closely related species.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035427 | PMC |
| http://dx.doi.org/10.1002/aps3.11323 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Total functioning capacity scale in Huntington's disease: natural course over time.
J Neurol
January 2025
LUMC Department of Neurology, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands.
Background And Objectives: The total functioning capacity (TFC) assessment has been integral to Huntington's disease (HD) research and clinical trials, measuring disease stage and progression. This study investigates the natural progression of function in HD, focusing on changes in TFC scores related to age and CAG-repeat length, and evaluates TFC's strengths and weaknesses in longitudinal studies.
Methods: Using Enroll-HD platform's clinical dataset version 5, including Registry-3, we analysed data from 21,079 participants, with 16,083 having an expanded CAG repeat.
Bioinformatics Analysis Reveals Microrchidia Family Genes as the Prognostic and Therapeutic Markers for Colorectal Cancer.
Endocr Metab Immune Disord Drug Targets
January 2025
Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, Taizhou, Zhejiang, China.
Aim: The aim of this study is to examine the role of the microrchidia (MORC) family, a group of chromatin remodeling proteins, as the therapeutic and prognostic markers for colorectal cancer (CRC).
Background: MORC protein family genes are a highly conserved nucleoprotein superfamily whose members share a common domain but have distinct biological functions. Previous studies have analyzed the roles of MORCs as epigenetic regulators and chromatin remodulators; however, the involvement of MORCs in the development and pathogenesis of CRC was less examined.
[Development of DNA molecular identity cards for germplasm of Murraya paniculata based on SSR markers].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center,China Academy of Chinese Medical Sciences Beijing 100700, China.
To promote the conservation and utilization of the germplasm resources and provide a basis for the breeding of new varieties of Murraya paniculata, this study analyzed the genetic diversity of the germplasm resources and developed the molecular identity(ID) card of M. paniculata. Multiple fluorescence PCR-capillary electrophoresis was performed for 65 germplasm accessions of M.
View Article and Find Full Text PDF[Establishment and application of chloroplast genome database with the largest number of species in world].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
The chloroplast genome is an important tool for studying plant classification, evolution, and the heterologous production of secondary metabolites and protein drugs. With advancements in sequencing technology and reductions in sequencing costs, chloroplast genome data have rapidly accumulated. However, existing chloroplast genome databases suffer from issues such as incomplete data, inadequate management, and inconsistent, inaccurate information, posing significant challenges for the development and utilization of the chloroplast genome.
View Article and Find Full Text PDFATXN2 polyglutamine intermediate repeats length expansions in Malaysian patients with amyotrophic lateral sclerosis (ALS).
Neurogenetics
January 2025
Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia.
Intermediate CAG repeats from 29 to 33 in the ATXN2 gene contributes to the risk of amyotrophic lateral sclerosis (ALS) in European and Asian populations. In this study, 148 ALS patients of multiethnic descent: Chinese (56.1%), Malay (24.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!