A novel electrochemiluminescence (ECL) biosensor was developed in this study, which was based on the Ag-NP modified tetrahedral DNA nanostructure. First, a stable and rigid three-dimensional tetrahedral DNA nanostructure (TDN) was modified on a gold electrode, which was used as a capture element. The TDN improves the accuracy and sensitivity of this biosensor. In addition, silver nanoparticles (Ag NPs) with excellent electrical conductivity were introduced on the edges of TDNs to enhance the ECL intensity of the metal-organic framework (MOF) nanomaterial. Apart from imparting conductivity, the Ag NPs can also act as co-reactant accelerators to enhance the ECL intensity. A Eu3+ doped Zr-MOF material (Eu@MOF) was proposed and used as an ECL material for the first time. Terminal phosphate-modified MUC1 aptamer DNA bonded with the exposed Zr nodes on the surface of MOFs through Zr-O-P bonds to construct the DNA-Eu@MOF signal element. Based on the Ag NP-modified TDN capture element and the DNA-Eu@MOF signal element, a hypersensitive biosensor was established. Under the optimal conditions, this biosensor exhibited a wide detection range from 1.135 fg mL-1 to 0.1135 ng mL-1 and a low detection limit of 0.37 fg mL-1 (S/N = 3) toward the target MUC1. The biosensor also showed excellent stability and high selectivity for MUC1 detection.
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http://dx.doi.org/10.1039/c9tb02773d | DOI Listing |
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December 2024
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Anal Chem
December 2024
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Highly sensitive and accurate detection of disease biomarkers is of great importance for diagnosis, staging, and treatment of certain diseases. Herein, we report a novel electrochemical method for the quantification of miRNA biomarkers with DNA tetrahedrons as the signal reporters. Upon the initiation of DNA hairpin opening by miRNA at the electrode interface, the hidden click reaction group is exposed for the bioconjugation with a tetrahedral DNA nanostructure, which carries multiple electrochemical species.
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December 2024
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, 610041, China.
Extensive accumulation of senescent cells contributes to organismal aging, and slowing down the process of cellular senescence may ameliorate age-related pathologies. Targeted inhibition of the mechanistic target of rapamycin complex 1 (mTORC1) is found to suppress the conversion of cells to senescence. The regulatory-associated protein of mTOR (Raptor), a key component of mTORC1, has been implicated as important in the aging process, and its druggability deserves to be investigated.
View Article and Find Full Text PDFTalanta
December 2024
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Military Medical Sciences Academy, Tianjin, 300050, China. Electronic address:
A two-color fluorescent sensing strategy based on a functionalized tetrahedral DNAzyme nanotweezer (FTDN) was developed to detect ochratoxin A (OTA) utilizing the multifunctional properties of DNA nanotechnology. The FTDN enables rapid OTA detection directly through a Cy5 fluorescent group, modified to respond to the target signal. Additionally, FTDN exhibits DNAzyme cutting activity in the presence of Mg ions, enabling it to traverse DNA nanoflower-functionalized magnetic beads.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
December 2024
Department of Synthetic Biology and Immunology, National Institute of Chemistry, Ljubljana, Slovenia.
Versatile DNA and polypeptide-based structures have been designed based on complementary modules. However, polypeptides can also form higher oligomeric states. We investigated the introduction of tetrameric modules as a substitute for coiled-coil dimerization units used in previous modular nanostructures.
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