AI Article Synopsis
- This study presents a new method that combines nuclear sub-fractionation with advanced mass spectrometry to effectively measure and quantify proteins found in the nucleus of human cells.
- The researchers used this method to investigate how cells respond to the sudden breakdown of BET bromodomains, uncovering surprising changes in chromatin regulation.
- The approach is straightforward and allows for a broader examination of chromatin and genome regulators that were previously difficult to study.
Article Abstract
Transcription factors and other chromatin-associated proteins are difficult to quantify comprehensively. Here, we combine facile nuclear sub-fractionation with data-independent acquisition mass spectrometry to achieve rapid, sensitive, and highly parallel quantification of the nuclear proteome in human cells. We apply this approach to quantify the response to acute degradation of BET bromodomains, revealing unexpected chromatin regulatory dynamics. The method is simple and enables system-level study of previously inaccessible chromatin and genome regulators.
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| http://dx.doi.org/10.1016/j.celrep.2020.01.096 | DOI Listing |
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