sp. nov., isolated from traditional Chinese yogurt.

Int J Syst Evol Microbiol

College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.

Published: April 2020

Three Gram-stain-positive bacterial strains, designated X0750, X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 16S rRNA gene and three housekeeping genes (, and ), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), DNA-DNA hybridization (DDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750 was phylogenetically related to the type strains of , , , , , , and , having 94.4-100 % 16S rRNA gene sequence similarities, 76.7-90.0 %  gene sequence similarities, 88.9-99.4 %  gene sequence similarities and 77.6-92.8 %  gene sequence similarities, respectively. ANI, DDH and AAI values between strain X0750 and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8 % respectively, confirming that strain X0750 represents a novel species within the genus . Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is X0750(=NCIMB 15192=CCM 8924=LMG 31184=CCTCC AB 2018403).

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Source
http://dx.doi.org/10.1099/ijsem.0.004062DOI Listing

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