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sp. nov., isolated from a freshwater river. | LitMetric

sp. nov., isolated from a freshwater river.

Int J Syst Evol Microbiol

Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd. Nan-Tzu, Taiwan, ROC, Kaohsiung City 811.

Published: March 2020

AI Article Synopsis

  • - Strain TTM-71, isolated from a freshwater river in Taiwan, was identified as a novel species within a specific bacterial genus through detailed taxonomic methods, including phylogenetic analysis of its DNA.
  • - This strain demonstrated a high genetic similarity to certain related species, particularly showing 95.5% similarity to a strain known as SR 2-06, and had specific growth preferences such as optimal conditions at 20°C, pH 8, and salt presence.
  • - TTM-71 was characterized as a Gram-negative, aerobic, rod-shaped bacterium with unique fatty acid and lipid profiles, leading to its classification as a new species, proposed as sp. nov., with specific strain designations.

Article Abstract

Strain TTM-71, isolated from a freshwater river in Taiwan, was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-71 is affiliated with species in the genus . The 16S rRNA gene sequence similarity indicated that strain TTM-71 is closely related to species within the genus (94.7-95.5 % sequence similarity) and had a high sequence similarity with SR 2-06 (95.5 %). Strain TTM-71 showed 70.3 % average nucleotide identity and 24.9 % digital DNA-DNA hybridization identity with YT21. Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed beige-colored colonies. Optimal growth occurred at 20 °C, pH 8, and in the presence of 0.5 % NaCl. The major fatty acids of strain TTM-71 were iso-C, iso-C G and summed feature 3 (comprising C ω and/or C ω). The predominant hydroxy fatty acid was iso-C 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, four uncharacterized aminophospholipids, one uncharacterized aminolipid, one uncharacterized phospholipid and one uncharacterized lipid. The predominant polyamine was homospermidine. The only isoprenoid quinone was MK-7. Genomic DNA G+C content was 45.6 mol%. On the basis of the polyphasic evidence presented, strain TTM-71 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TTM-71 (=BCRC 81160=LMG 31017=KCTC 62871).

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Source
http://dx.doi.org/10.1099/ijsem.0.003875DOI Listing

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