tRNA molecules, which contain the most abundant post-transcriptional modifications, are crucial for proper gene expression and protein biosynthesis. Methylation at N1 of adenosine 58 (A58) is critical for maintaining the stability of initiator methionyl-tRNA (tRNAiMet) in bacterial, archaeal, and eukaryotic tRNAs. However, although research has been conducted in yeast and mammals, it remains unclear how A58 in plant tRNAs is modified and involved in development. In this study, we identify the nucleus-localized complex AtTRM61/AtTRM6 in Arabidopsis as tRNA m1A58 methyltransferase. Deficiency or a lack of either AtTRM61 or AtTRM6 leads to embryo arrest and seed abortion. The tRNA m1A level decreases in conditionally complemented Attrm61/LEC1pro::AtTRM61 plants and this is accompanied by reduced levels of tRNAiMet, indicating the importance of the tRNA m1A modification for tRNAiMet stability. Taken together, our results demonstrate that tRNA m1A58 modification is necessary for tRNAiMet stability and is required for embryo development in Arabidopsis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475180 | PMC |
| http://dx.doi.org/10.1093/jxb/eraa100 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Combining Nanopore direct RNA sequencing with genetics and mass spectrometry for analysis of T-loop base modifications across 42 yeast tRNA isoacceptors.
Nucleic Acids Res
October 2024
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Transfer RNAs (tRNAs) contain dozens of chemical modifications. These modifications are critical for maintaining tRNA tertiary structure and optimizing protein synthesis. Here we advance the use of Nanopore direct RNA-sequencing (DRS) to investigate the synergy between modifications that are known to stabilize tRNA structure.
View Article and Find Full Text PDFtRNA-mA methylation controls the infection of Magnaporthe oryzae by supporting ergosterol biosynthesis.
Dev Cell
November 2024
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
Article Synopsis
- Ergosterols are vital for fungal cell membranes, and targeting the genes responsible for their biosynthesis is key to managing fungal diseases like rice blast caused by Magnaporthe oryzae.
- The Trm6/Trm61 complex adds a specific modification (mA58) to tRNAs, which plays a crucial role in speeding up protein synthesis by enhancing the binding of tRNA to the ribosome during translation.
- Disrupting this mA58 process reduces the production of ergosterols and weakens fungal virulence, suggesting that a combined approach targeting both mA58 modification and ergosterol biosynthesis could be effective in developing new fungicides.
tRNA mA modification regulate HSC maintenance and self-renewal via mTORC1 signaling.
Nat Commun
July 2024
Zhejiang Key Laboratory of Medical Epigenetics, School of Basic Medical Sciences, The Third People's Hospital of Deqing, Department of Cardiology, Affiliated Hospital of Hangzhou Normal University, Hangzhou Normal University, Hangzhou, 311121, China.
Haematopoietic stem cells (HSCs) possess unique physiological adaptations to sustain blood cell production and cope with stress responses throughout life. To maintain these adaptations, HSCs rely on maintaining a tightly controlled protein translation rate. However, the mechanism of how HSCs regulate protein translation remains to be fully elucidated.
View Article and Find Full Text PDFHuman T-cell leukemia virus type 1 uses a specific tRNA isodecoder to prime reverse transcription.
RNA
July 2024
Department of Chemistry and Biochemistry, Center for RNA Biology, and Center for Retrovirus Research, The Ohio State University, Columbus, Ohio 43210, USA
Human T-cell leukemia virus type 1 (HTLV-1) is the only oncogenic human retrovirus discovered to date. All retroviruses are believed to use a host cell tRNA to prime reverse transcription (RT). In HTLV-1, the primer-binding site (PBS) in the genomic RNA is complementary to the 3' 18 nucleotides (nt) of human tRNA The human genome encodes 20 cytoplasmic tRNA genes representing seven isodecoders, all of which share the same 3' 18 nt sequence but vary elsewhere.
View Article and Find Full Text PDFAdvances in the Structural and Functional Understanding of mA RNA Modification.
Acc Chem Res
February 2024
Université Paris Cité, CNRS, Institut de Biologie Physico-Chimique, IBPC, Expression Génétique Microbienne, Paris 75005, France.
ConspectusRNA modification is a co- or post-transcriptional process by which specific nucleotides are chemically altered by enzymes after their initial incorporation into the RNA chain, expanding the chemical and functional diversity of RNAs. Our understanding of RNA modifications has changed dramatically in recent years. In the past decade, RNA methyltransferases (MTases) have been highlighted in numerous clinical studies and disease models, modifications have been found to be dynamically regulated by demodification enzymes, and significant technological advances have been made in the fields of RNA sequencing, mass spectrometry, and structural biology.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!