AI Article Synopsis
- Kinetoplastids use post-transcriptional regulation for gene expression control, involving RNA-binding proteins for mRNA processes.
- ERBP1 and ERBP2 are identified as mRNA-binding proteins associated with the endoplasmic reticulum, with ERBP1 showing interaction with ERBP2.
- Loss of ERBP1 leads to initial growth defects in bloodstream-form Kinetoplastids, but growth is eventually restored; ERBP1 preferentially binds ribosomal protein mRNAs and does not associate with secreted protein mRNAs or stress granules.
Article Abstract
Kinetoplastids rely heavily on post-transcriptional mechanisms for control of gene expression, and on RNA-binding proteins that regulate mRNA splicing, translation and decay. ERBP1 (Tb927.10.14150) and ERBP2 (Tb927.9.9550) were previously identified as mRNA binding proteins that lack canonical RNA-binding domains. We show here that ERBP1 is associated with the endoplasmic reticulum, like ERBP2, and that the two proteins interact in vivo. Loss of ERBP1 from bloodstream-form initially resulted in a growth defect but proliferation was restored after more prolonged cultivation. Pull-down analysis of tagged ERBP1 suggests that it preferentially binds to ribosomal protein mRNAs. The ERBP1 sequence resembles that of Bfr1, which also localises to the endoplasmic reticulum and binds to ribosomal protein mRNAs. However, unlike Bfr1, ERBP1 does not bind to mRNAs encoding secreted proteins, and it is also not recruited to stress granules after starvation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025706 | PMC |
| http://dx.doi.org/10.7717/peerj.8388 | DOI Listing |
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